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USMG5 Polyclonal antibody

USMG5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17716-1-AP

Print datasheet

Synonyms

HCV F transactivated protein 2, HCVFTP2, USMG5

验证数据展示

  • IHC staining of mouse brain using 17716-1-AP

    IHC staining of mouse brain using 17716-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17716-1-AP (USMG5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 17716-1-AP

    IHC staining of mouse brain using 17716-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17716-1-AP (USMG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 17716-1-AP

    WB analysis using 17716-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17716-1-AP (USMG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, human liver tissue, human brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17716-1-AP targets USMG5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Immunogen USMG5 fusion protein Ag12086 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name up-regulated during skeletal muscle growth 5 homolog (mouse)
Synonyms HCV F transactivated protein 2, HCVFTP2, USMG5
Calculated Molecular Weight 58 aa, 7 kDa
Observed Molecular Weight 6 kDa
GenBank Accession NumberBC072683
Gene Symbol USMG5
Gene ID (NCBI) 84833
RRIDAB_2214288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96IX5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for USMG5 antibody 17716-1-APDownload protocol
IHC protocol for USMG5 antibody 17716-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Clin Invest

Deficiency in Kelch protein Klhl31 causes congenital myopathy in mice.

Authors - James B Papizan
View Article
mouseIF

Sci Rep

miR-379 links glucocorticoid treatment with mitochondrial response in Duchenne muscular dystrophy.

Authors - M Sanson
View Article
ratWB

Mol Psychiatry

Proteasome-independent K63 polyubiquitination selectively regulates ATP levels and proteasome activity during fear memory formation in the female amygdala

Authors - Kayla Farrell
View Article