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- KD/KO Validated
USP1 Polyclonal antibody
USP1 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IP, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells |
Positive IP detected in | HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14346-1-AP targets USP1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP |
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat |
Immunogen | USP1 fusion protein Ag5686 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ubiquitin specific peptidase 1 |
Synonyms | Ubiquitin thioesterase 1, Ubiquitin carboxyl-terminal hydrolase 1, N-terminal fragment, Ubiquitin carboxyl-terminal hydrolase 1, hUBP, EC:3.4.19.12 |
Calculated Molecular Weight | 88 kDa |
Observed Molecular Weight | 110 kDa, 100 kDa |
GenBank Accession Number | BC050525 |
Gene Symbol | USP1 |
Gene ID (NCBI) | 7398 |
RRID | AB_2214314 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O94782 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for USP1 antibody 14346-1-AP | Download protocol |
IP protocol for USP1 antibody 14346-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Chem Biol A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses. | ||
Mol Cell Polyubiquitinated PCNA recruits the ZRANB3 translocase to maintain genomic integrity after replication stress. | ||
Nat Commun The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites. | ||
Nat Commun hnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma. | ||
Theranostics CRISPR/Cas9-based genome-wide screening for deubiquitinase subfamily identifies USP1 regulating MAST1-driven cisplatin-resistance in cancer cells |