• Featured Product
  • KD/KO Validated

USP1 Polyclonal antibody

USP1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 14346-1-AP

Print datasheet

Synonyms

Ubiquitin thioesterase 1, Ubiquitin carboxyl-terminal hydrolase 1, N-terminal fragment, Ubiquitin carboxyl-terminal hydrolase 1, hUBP, EC:3.4.19.12



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14346-1-AP targets USP1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen USP1 fusion protein Ag5686 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin specific peptidase 1
Synonyms Ubiquitin thioesterase 1, Ubiquitin carboxyl-terminal hydrolase 1, N-terminal fragment, Ubiquitin carboxyl-terminal hydrolase 1, hUBP, EC:3.4.19.12
Calculated Molecular Weight 88 kDa
Observed Molecular Weight 110 kDa, 100 kDa
GenBank Accession NumberBC050525
Gene Symbol USP1
Gene ID (NCBI) 7398
RRIDAB_2214314
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94782
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP1(Ubiquitin carboxyl-terminal hydrolase 1) is a negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. It is also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. This protein, which consists of 785 amino acids with a deduced molecular mass of 88.2 kDa, possesses His and Cys domains that are highly conserved in all members of the ubiquitin-specific processing (UBP) family of proteases (PMID: 9806842). The USP1 enzyme can undergo autocleavage, resulting in a 100 kDa N-terminal fragment and a 14 kDa C-terminal fragment (PMID: 18082604).

实验方案

Product Specific Protocols
WB protocol for USP1 antibody 14346-1-APDownload protocol
IP protocol for USP1 antibody 14346-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Chem Biol

A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses.

Authors - Qin Liang
humanWB

Mol Cell

Polyubiquitinated PCNA recruits the ZRANB3 translocase to maintain genomic integrity after replication stress.

Authors - Ciccia Alberto A
humanWB,IF

Nat Commun

The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites.

Authors - Kyungsoo Ha
humanWB

Nat Commun

hnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma.

Authors - Pauline Herviou
humanWB

Cancer Immunol Res

USP22 deubiquitinates CD274 to suppress anti-cancer immunity.

Authors - Xing Huang
humanWB,IHC,IF,IP

Theranostics

CRISPR/Cas9-based genome-wide screening for deubiquitinase subfamily identifies USP1 regulating MAST1-driven cisplatin-resistance in cancer cells

Authors - Apoorvi Tyagi