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验证数据展示

  • WB analysis using 81435-2-RR

    WB analysis using 81435-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81435-2-RR (USP10 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 81435-2-RR

    WB analysis of NIH/3T3 using 81435-2-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81435-2-RR (USP10 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 81435-2-RR

    IF Staining of HepG2 using 81435-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using USP10 antibody (81435-2-RR, Clone: 240583G6 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

USP10 Recombinant antibody

USP10 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 81435-2-RR

产品说明书

CloneNo. 240583G6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Ubiquitin-specific-processing protease 10, Ubiquitin carboxyl-terminal hydrolase 10, KIAA0190, EC:3.4.19.12, Deubiquitinating enzyme 10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, NIH/3T3 cells, HEK-293 cells, A549 cells, LNCaP cells, K-562 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81435-2-RR targets USP10 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 USP10 fusion protein Ag7233 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin specific peptidase 10
别名 Ubiquitin-specific-processing protease 10, Ubiquitin carboxyl-terminal hydrolase 10, KIAA0190, EC:3.4.19.12, Deubiquitinating enzyme 10
计算分子量 87 kDa, 92 kDa
观测分子量 100-130 kDa
GenBank蛋白编号BC000263
基因名称 USP10
Gene ID (NCBI) 9100
RRIDAB_3670495
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ14694
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP10, also named as USP32 and NY-REN-60, belongs to the peptidase C19 family.The deubiquitinating protease USP10 has been identified as a new regulator of p53 in the DNA damage response and tumor development.

实验方案

Product Specific Protocols
WB protocol for USP10 antibody 81435-2-RRDownload protocol
IF protocol for USP10 antibody 81435-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols