- Featured Product
- KD/KO Validated
USP11 Polyclonal antibody
USP11 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HEK-293 cells, human testis tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy USP11 Ready-To-Use IHC Kit. USP11 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 5 publications below |
| WB | See 9 publications below |
| IHC | See 4 publications below |
产品信息
10244-1-AP targets USP11 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | USP11 fusion protein Ag0296 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ubiquitin specific peptidase 11 |
| Synonyms | Deubiquitinating enzyme 11, Ubiquitin thiolesterase 11, UHX1, USP11 |
| Calculated Molecular Weight | 963 aa, 110 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC000350 |
| Gene Symbol | USP11 |
| Gene ID (NCBI) | 8237 |
| RRID | AB_2288400 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51784 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
USP11(Ubiquitin carboxyl-terminal hydrolase 11), also named as UHX1, is a deubiquitinating enzyme controling many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. There are four bands of 120 kDa, 55 kDa, 50 kDa and 44 kDa to be detected in HeLa extract by western blot and the smaller proteins may be degradation products, some other spliced products could possibly correspond to the smaller proteins and possess functions(PMID:12084015). This protein belongs to the peptidase C19 family.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USP11 antibody 10244-1-AP | Download protocol |
| IHC protocol for USP11 antibody 10244-1-AP | Download protocol |
| IP protocol for USP11 antibody 10244-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol XRCC1 protects transcription from toxic PARP1 activity during DNA base excision repair. | ||
Theranostics A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis. | ||
Clin Transl Med EGFR-vIII downregulated H2AZK4/7AC though the PI3K/AKT-HDAC2 axis to regulate cell cycle progression. | ||
Oncogene The deubiquitinase USP11 regulates cell proliferation and ferroptotic cell death via stabilization of NRF2 USP11 deubiquitinates and stabilizes NRF2. | ||
Cancer Lett The E2F1/USP11 positive feedback loop promotes hepatocellular carcinoma metastasis and inhibits autophagy by activating ERK/mTOR pathway.
| ||
Int J Radiat Oncol Biol Phys USP11 exacerbates radiation induced pneumonitis by activating endothelial cell inflammatory response via OTUD5-STING signaling |
