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  • Featured Product
  • KD/KO Validated

USP11 Monoclonal antibody

USP11 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G6E7

Cat no : 67805-1-Ig

Print datasheet

Synonyms

Ubiquitin-specific-processing protease 11, Ubiquitin thiolesterase 11, Ubiquitin thioesterase 11, Ubiquitin carboxyl-terminal hydrolase 11, EC:3.4.19.12

验证数据展示

  • WB analysis using 67805-1-Ig

    WB analysis using 67805-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67805-1-Ig (USP11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 67805-1-Ig

    WB analysis of HEK-293 using 67805-1-Ig

    WB result of USP11 antibody (67805-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP11 transfected HEK-293 cells.

  • IF Staining of HepG2 using 67805-1-Ig

    IF Staining of HepG2 using 67805-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using USP11 antibody (67805-1-Ig, Clone: 2G6E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human stomach cancer using 67805-1-Ig

    IHC staining of human stomach cancer using 67805-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67805-1-Ig (USP11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with USP11 antibody included
  • IHC staining of rat testis using 67805-1-Ig

    IHC staining of rat testis using 67805-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67805-1-Ig (USP11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with USP11 antibody included

经过测试的应用

Positive WB detected inHCT 116 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, Ramos cells, Daudi cells
Positive IHC detected inrat testis tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy USP11 Ready-To-Use IHC Kit. USP11 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67805-1-Ig targets USP11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen USP11 fusion protein Ag19058 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin specific peptidase 11
Synonyms Ubiquitin-specific-processing protease 11, Ubiquitin thiolesterase 11, Ubiquitin thioesterase 11, Ubiquitin carboxyl-terminal hydrolase 11, EC:3.4.19.12
Calculated Molecular Weight 963 aa, 110 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberBC000350
Gene Symbol USP11
Gene ID (NCBI) 8237
RRIDAB_2918568
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP51784
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP11(Ubiquitin carboxyl-terminal hydrolase 11), also named as UHX1, is a deubiquitinating enzyme controling many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. There are four bands of 120 kDa, 55 kDa, 50 kDa and 44 kDa to be detected in HeLa extract by western blot and the smaller proteins may be degradation products, some other spliced products could possibly correspond to the smaller proteins and possess functions(PMID:12084015). This protein belongs to the peptidase C19 family.

实验方案

Product Specific Protocols
WB protocol for USP11 antibody 67805-1-IgDownload protocol
IHC protocol for USP11 antibody 67805-1-IgDownload protocol
IF protocol for USP11 antibody 67805-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols