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验证数据展示

  • WB analysis of HeLa using 66176-1-Ig (same clone as 66176-1-PBS)

    WB analysis of HeLa using 66176-1-Ig (same clone as 66176-1-PBS)

    WB result of USP13 antibody (66176-1-Ig, 1:600) with si-Control and si-USP13 transfected HeLa cells. This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

  • WB analysis using 66176-1-Ig (same clone as 66176-1-PBS)

    WB analysis using 66176-1-Ig (same clone as 66176-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66176-1-Ig (USP13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66176-1-Ig (same clone as 66176-1-PBS)

    IHC staining of human testis using 66176-1-Ig (same clone as 66176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66176-1-Ig (same clone as 66176-1-PBS)

    IHC staining of human testis using 66176-1-Ig (same clone as 66176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66176-1-Ig (same clone as 66176-1-PBS)

    IF Staining of HepG2 using 66176-1-Ig (same clone as 66176-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66176-1-Ig (USP13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66176-1-Ig (same clone as 66176-1-PBS)

    IF Staining of HepG2 using 66176-1-Ig (same clone as 66176-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using USP13 antibody (66176-1-Ig, Clone: 6E8C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66176-1-Ig (same clone as 66176-1-PBS)

    WB analysis of HepG2 using 66176-1-Ig (same clone as 66176-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66176-1-Ig (USP13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

USP13 Monoclonal antibody, PBS Only

USP13 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66176-1-PBS

产品说明书

CloneNo. 6E8C10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

6E8C10, EC:3.4.19.12, Isopeptidase T-3, ISOT3, ISOT-3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66176-1-PBS targets USP13 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10506

Product name: Recombinant human USP13 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 515-863 aa of BC016146

Sequence: IAYELTRREAEANRRPLPELVRAKIPFSACLQAFSEPENVDDFWSSALQAKSAGVKTSRFASFPEYLVVQIKKFTFGLDWVPKKFDVSIDMPDLLDINHLRARGLQPGEEELPDISPPIVIPDDSKDRLMNQLIDPSDIDESSVMQLAEMGFPLEACRKAVYFTGNMGAEVAFNWIIVHMEEPDFAEPLTMPGYGGAASAGASVFGASGLDNQPPEEIVAIITSMGFQRNQAIQALRATNNNLERALDWIFSHPEFEEDSDFVIEMENNANANIISEAKPEGPRVKDGSGTYELFAFISHMGTSTMSGHYICHIKKEGRWVIYNDHKVCASERPPKDLGYMYFYRRIPS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 13 (isopeptidase T-3)
别名 6E8C10, EC:3.4.19.12, Isopeptidase T-3, ISOT3, ISOT-3
计算分子量 863 aa, 97 kDa
观测分子量97-100 kDa
GenBank蛋白编号BC016146
基因名称 USP13
Gene ID (NCBI) 8975
RRIDAB_2881571
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ92995
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP13(Ubiquitin carboxyl-terminal hydrolase 13) is also named as ISOT3 and belongs to the peptidase C19 family. It is involved in ubiquitin-dependent protein catabolism. USP13 is responsible for MITF deubiquitination, utilizing a short hairpin RNA library against known deubiquitinating enzymes. This protein has 2 isoforms produced by alternative splicing.

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