验证数据展示
经过测试的应用
| Positive WB detected in | DU 145 cells, HeLa cells, mouse heart tissue, HEK-293T cells, HEK-293 cells, SKOV-3 cells, Neuro-2a cells |
| Positive IP detected in | mouse liver tissue |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 555 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:18000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14517-1-AP targets USP14 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 | USP14 fusion protein Ag5979 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) |
| 别名 | Ubiquitin thiolesterase 14, Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC:3.4.19.12 |
| 计算分子量 | 54 kDa |
| 观测分子量 | 52-60 kDa |
| GenBank蛋白编号 | BC003556 |
| 基因名称 | USP14 |
| Gene ID (NCBI) | 9097 |
| RRID | AB_2257124 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P54578 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.This protein has a 45-kDa catalytic domain (PMID:16211010).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USP14 antibody 14517-1-AP | Download protocol |
| IF protocol for USP14 antibody 14517-1-AP | Download protocol |
| IP protocol for USP14 antibody 14517-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy USP14 regulates DNA damage repair by targeting RNF168-dependent ubiquitination. | ||
Autophagy Inhibition of USP14 influences alphaherpesvirus proliferation by degrading viral VP16 protein via ER stress-triggered selective autophagy.
| ||
Cell Prolif SPAG5-AS1 inhibited autophagy and aggravated apoptosis of podocytes via SPAG5/AKT/mTOR pathway. | ||
Front Pharmacol SOAT1 Promotes Gastric Cancer Lymph Node Metastasis Through Lipid Synthesis. | ||
J Biol Chem The USP14-NLRC5 pathway inhibits titanium particle-induced osteolysis in mice by suppressing NF-κB and PI3K/AKT activities. | ||
Oral Dis USP14 promotes the cancer stem-like cell properties of OSCC via promoting SOX2 deubiquitination
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