验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, HeLa cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, U2OS cells, K-562 cells |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67746-1-Ig targets USP14 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | Human, Mouse, Rat, Pig, Rabbit |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | USP14 fusion protein Ag6312 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) |
| 别名 | Deubiquitinating enzyme 14, TGT, Ubiquitin thiolesterase 14, USP14 |
| 计算分子量 | 54 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC003556 |
| 基因名称 | USP14 |
| Gene ID (NCBI) | 9097 |
| RRID | AB_2918515 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P54578 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USP14 antibody 67746-1-Ig | Download protocol |
| IF protocol for USP14 antibody 67746-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oral Dis USP14 promotes the cancer stem-like cell properties of OSCC via promoting SOX2 deubiquitination | ||
Redox Biol ALDH2 deficiency augments atherosclerosis through the USP14-cGAS-dependent polarization of proinflammatory macrophages | ||
Int J Mol Sci Targeting USP14/UCHL5: A Breakthrough Approach to Overcoming Treatment-Resistant FLT3-ITD-Positive AML | ||
Free Radic Biol Med USP14 modulates cell pyroptosis and ameliorates doxorubicin-induced cardiotoxicity by deubiquitinating and stabilizing SIRT3 | ||
Br J Cancer GSTM3 enhances radiosensitivity of nasopharyngeal carcinoma by promoting radiation-induced ferroptosis through USP14/FASN axis and GPX4 |