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USP15 Polyclonal antibody
USP15 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, mouse brain tissue, HepG2 cells, K-562 cells, MCF-7 cells, NIH/3T3 cells, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human breast cancer tissue, human ovary tumor tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14354-1-AP targets USP15 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, bovine |
Immunogen | USP15 fusion protein Ag5703 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ubiquitin specific peptidase 15 |
Synonyms | Deubiquitinating enzyme 15, KIAA0529, Ubiquitin thiolesterase 15, Unph 2, UNPH4, USP15 |
Calculated Molecular Weight | 112 kDa |
Observed Molecular Weight | 112 kDa |
GenBank Accession Number | BC020688 |
Gene Symbol | USP15 |
Gene ID (NCBI) | 9958 |
RRID | AB_2257148 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y4E8 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for USP15 antibody 14354-1-AP | Download protocol |
IHC protocol for USP15 antibody 14354-1-AP | Download protocol |
IP protocol for USP15 antibody 14354-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Nat Immunol USP15 stabilizes MDM2 to mediate cancer-cell survival and inhibit antitumor T cell responses.
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Nat Commun ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy
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Autophagy USP33 deubiquitinates PRKN/parkin and antagonizes its role in mitophagy.
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Autophagy LncRNA 2810403D21Rik/Mirf promotes ischemic myocardial injury by regulating autophagy through targeting Mir26a. | ||
Leukemia The deubiquitinase USP15 modulates cellular redox and is a therapeutic target in acute myeloid leukemia | ||
Sci Total Environ USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.
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