验证数据展示

WB analysis of mouse brain using 14354-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14354-1-AP (USP15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 14354-1-AP
IP result of anti-USP15 (IP:14354-1-AP, 4ug; Detection:14354-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of human breast cancer using 14354-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14354-1-AP (USP15 Antibody) at dilution of 1:200 (under 10x lens).
Ready-to-use IHC kits available with USP15 antibody included
IHC staining of human breast cancer using 14354-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14354-1-AP (USP15 Antibody) at dilution of 1:200 (under 40x lens).
Ready-to-use IHC kits available with USP15 antibody included
WB analysis using 14354-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14354-1-AP (USP15 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 14354-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14354-1-AP (USP15 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IHC staining of human placenta using 14354-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14354-1-AP (USP15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with USP15 antibody included
IHC staining of human lung using 14354-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14354-1-AP (USP15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with USP15 antibody included
IHC staining of mouse kidney using 14354-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14354-1-AP (USP15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with USP15 antibody included
IHC staining of rat small intestine using 14354-1-AP
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 14354-1-AP (USP15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with USP15 antibody included

Featured Product
KD/KO验证

USP15 Polyclonal antibody

USP15 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 14354-1-AP

产品说明书

引用文献(21)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IP, ELISA and More (2)

别名

Ubiquitin thiolesterase 15, Ubiquitin thioesterase 15, Ubiquitin carboxyl-terminal hydrolase 15, KIAA0529, EC:3.4.19.12

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (21)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HEK-293 cells, HeLa cells, mouse brain tissue, HepG2 cells, K-562 cells, MCF-7 cells, NIH/3T3 cells, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human placenta tissue, human breast cancer tissue, human lung tissue, human ovary tumor tissue, mouse kidney tissue, rat small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Planning an IHC experiment? We recommend our IHCeasy USP15 Ready-To-Use IHC Kit. USP15 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 11 publications below
WB	See 18 publications below
IHC	See 2 publications below
IF	See 5 publications below
IP	See 2 publications below
CoIP	See 2 publications below

产品信息

14354-1-AP targets USP15 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, bovine
免疫原	USP15 fusion protein Ag5703 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	ubiquitin specific peptidase 15
别名	Ubiquitin thiolesterase 15, Ubiquitin thioesterase 15, Ubiquitin carboxyl-terminal hydrolase 15, KIAA0529, EC:3.4.19.12
计算分子量	112 kDa
观测分子量	112 kDa
GenBank蛋白编号	BC020688
基因名称	USP15
Gene ID (NCBI)	9958
RRID	AB_2257148
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q9Y4E8
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Ubiquitin is a highly conserved eukaryotic protein that is synthesized as a fusion protein precursor, either to itself or to one of two ribosomal proteins. USP15, is also named as KIAA0529,Unph-2, Unph4, encoding a human ubiquitin-specific protease (USP). The USP15 protein contains the highly conserved Cys and His boxes present in all members of he UBP family of deubiquitinating enzymes(PMID:10444327).It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for USP15 antibody 14354-1-AP	Download protocol
IHC protocol for USP15 antibody 14354-1-AP	Download protocol
IP protocol for USP15 antibody 14354-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Nat Immunol USP15 stabilizes MDM2 to mediate cancer-cell survival and inhibit antitumor T cell responses. Authors - Qiang Zou KO Validated View Article
human,mouse	WB	Nat Commun ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy Authors - Xiangling Xiao KD Validated View Article
mouse	WB	Mol Cell RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages Authors - Teodorus Theo Susanto View Article
human	WB	Autophagy USP33 deubiquitinates PRKN/parkin and antagonizes its role in mitophagy. Authors - Kaifeng Niu KD Validated View Article
mouse	WB	Autophagy LncRNA 2810403D21Rik/Mirf promotes ischemic myocardial injury by regulating autophagy through targeting Mir26a. Authors - Haihai Liang View Article
human	WB	Leukemia The deubiquitinase USP15 modulates cellular redox and is a therapeutic target in acute myeloid leukemia Authors - Madeline Niederkorn View Article

验证数据展示

WB analysis of mouse brain using 14354-1-AP

IP experiment of mouse brain using 14354-1-AP

IHC staining of human breast cancer using 14354-1-AP

IHC staining of human breast cancer using 14354-1-AP

WB analysis using 14354-1-AP

WB analysis using 14354-1-AP

IHC staining of human placenta using 14354-1-AP

IHC staining of human lung using 14354-1-AP

IHC staining of mouse kidney using 14354-1-AP

IHC staining of rat small intestine using 14354-1-AP

USP15 Polyclonal antibody

USP15 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

USP15 stabilizes MDM2 to mediate cancer-cell survival and inhibit antitumor T cell responses.

ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy

RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages

USP33 deubiquitinates PRKN/parkin and antagonizes its role in mitophagy.

LncRNA 2810403D21Rik/Mirf promotes ischemic myocardial injury by regulating autophagy through targeting Mir26a.

The deubiquitinase USP15 modulates cellular redox and is a therapeutic target in acute myeloid leukemia