验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21362-1-AP targets USP17 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | USP17 fusion protein Ag15824 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin specific peptidase 17 |
| 别名 | RS447, DUB-3 , DUB3 |
| 计算分子量 | 530 aa, 60 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC100991 |
| 基因名称 | USP17 |
| Gene ID (NCBI) | 391627 |
| RRID | AB_3669365 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
USP17, also known as DUB3, is a deubiquitinating enzyme (DUB) that plays a key role in regulating the balance between ubiquitination and deubiquitination of proteins. This balance is essential for protein degradation, transport, localization, and activity. USP17 is inducibly expressed in response to cytokine, chemokine, and epidermal growth factor (EGF) stimulation, and several studies have shown that it is required for cell proliferation and migration. Aberrantly expressed USP17 has been associated with inflammation, cell motility, development of T helper 17 (Th17) cells and carcinogenesis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USP17 antibody 21362-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |