验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, Jurkat cells, MDA-MB-231 cells |
| Positive IP detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25768-1-AP targets USP19 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | USP19 fusion protein Ag22646 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin specific peptidase 19 |
| 别名 | Deubiquitinating enzyme 19, KIAA0891, Ubiquitin thiolesterase 19, USP19, ZMYND9 |
| 计算分子量 | 1318 aa, 146 kDa |
| 观测分子量 | 70 kDa, 120 kDa, 150-180 kDa |
| GenBank蛋白编号 | BC146752 |
| 基因名称 | USP19 |
| Gene ID (NCBI) | 10869 |
| RRID | AB_2713918 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O94966 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ubiquitin-specific protease (USP)19 is a recently identified deubiquitinating enzyme (DUB) having multiple splice variants and cellular functions. Since the sum of molecular weights of the two small bands (65 kDa+120 kDa) approximates the size of the full-length protein (~185 kDa), USP19 is likely to be endoproteolytically cleaved. Interestingly, the small bands were not observed when the DUB activity was inactivated, suggesting that the cleavage of USP19 depends on its DUB activity (PMID: 25088257).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USP19 antibody 25768-1-AP | Download protocol |
| IP protocol for USP19 antibody 25768-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Clin Transl Med Deubiquitinase USP19 modulates apoptotic calcium release and endoplasmic reticulum stress by deubiquitinating BAG6 in triple negative breast cancer | ||
Mol Ther Dual role of deubiquitinating enzyme USP19 regulates mitotic progression and tumorigenesis by stabilizing survivin
| ||
J Cell Biol USP19 promotes hypoxia-induced mitochondrial division via FUNDC1 at ER-mitochondria contact sites. | ||
Theranostics USP32 confers cancer cell resistance to YM155 via promoting ER-associated degradation of solute carrier protein SLC35F2
| ||
Cell Biol Toxicol Deubiquitinase USP19 enhances phenylalanine hydroxylase protein stability and its enzymatic activity.
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