USP2 Polyclonal antibody
USP2 Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, MCF-7 cells, mouse brain tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| CoIP | See 1 publications below |
产品信息
15404-1-AP targets USP2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF, IHC, ELISA Application Description |
| Cited Applications | WB, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | USP2 fusion protein Ag7658 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ubiquitin specific peptidase 2 |
| Synonyms | Deubiquitinating enzyme 2, ubiquitin specific peptidase 2, Ubiquitin thiolesterase 2, UBP41, USP2, USP9 |
| Calculated Molecular Weight | 68 kDa |
| Observed Molecular Weight | 41-50 kDa, 68 kDa |
| GenBank Accession Number | BC002854 |
| Gene Symbol | USP2 |
| Gene ID (NCBI) | 9099 |
| RRID | AB_10596634 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75604 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ubiquitin, a highly conserved protein involved in the regulation of intracellular protein breakdown, cell cycle regulation, and stress response, is released from degraded proteins by disassembly of the polyubiquitin chains. The disassembly process is mediated by ubiquitin-specific proteases (USPs). USP2 is also named as UBP41. USP2 can interact indirectly with multiple clock proteins, so it remains possible that USP2 may modulate the ubiquitination and function of these other proteins. It has 4 isoforms produced by alternative splicing with the MW of 68, 41, 40 and 45 kDa. USP2 can exsit as a homooligomer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USP2 antibody 15404-1-AP | Download protocol |
| IHC protocol for USP2 antibody 15404-1-AP | Download protocol |
| IF protocol for USP2 antibody 15404-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Transl Oncol The role of deubiquitinase USP2 in driving bladder cancer progression by stabilizing EZH2 to epigenetically silence SOX1 expression
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