验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse lung tissue, rat lung tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13126-1-AP targets USP24 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | USP24 fusion protein Ag3779 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ubiquitin specific peptidase 24 |
| Synonyms | Ubiquitin thiolesterase 24, Ubiquitin thioesterase 24, Ubiquitin carboxyl-terminal hydrolase 24, KIAA1057, EC:3.4.19.12 |
| Calculated Molecular Weight | 294 kDa |
| Observed Molecular Weight | 280-294 kDa |
| GenBank Accession Number | BC029660 |
| Gene Symbol | USP24 |
| Gene ID (NCBI) | 23358 |
| RRID | AB_2212761 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UPU5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USP24 antibody 13126-1-AP | Download protocol |
| IHC protocol for USP24 antibody 13126-1-AP | Download protocol |
| IF protocol for USP24 antibody 13126-1-AP | Download protocol |
| IP protocol for USP24 antibody 13126-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer LINE-1 promotes tumorigenicity and exacerbates tumor progression via stimulating metabolism reprogramming in non-small cell lung cancer | ||
Nat Commun USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and β-TrCP and promotes cancer malignancy.
| ||
Nat Commun A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation.
| ||
Nat Commun ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP. | ||
EMBO Mol Med USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma. |
