- Featured Product
- KD/KO Validated
USP25 Polyclonal antibody
USP25 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | BxPC-3 cells, mouse cerebellum tissue, K-562 cells |
Positive IP detected in | RAW 264.7 cells |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 2 publications below |
WB | See 5 publications below |
IHC | See 1 publications below |
产品信息
12199-1-AP targets USP25 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | USP25 fusion protein Ag2837 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ubiquitin specific peptidase 25 |
Synonyms | Deubiquitinating enzyme 25, Ubiquitin thiolesterase 25, USP on chromosome 21, USP21, USP25 |
Calculated Molecular Weight | 125 kDa |
Observed Molecular Weight | 125 kDa |
GenBank Accession Number | BC015930 |
Gene Symbol | USP25 |
Gene ID (NCBI) | 29761 |
RRID | AB_2212771 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9UHP3 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for USP25 antibody 12199-1-AP | Download protocol |
IHC protocol for USP25 antibody 12199-1-AP | Download protocol |
IP protocol for USP25 antibody 12199-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Death Dis Suppression of USP7 induces BCR-ABL degradation and chronic myelogenous leukemia cell apoptosis. | ||
Biochem Pharmacol Inhibition of deubiquitinase USP28 attenuates cyst growth in autosomal dominant polycystic kidney disease | ||
Nucleic Acids Res The dimeric deubiquitinase USP28 integrates 53BP1 and MYC functions to limit DNA damage | ||
Ren Fail USP25 attenuates anti-GBM nephritis in mice by negative feedback regulation of Th17 cell differentiation
| ||
J Cell Physiol The miR-27a-3p/USP25 axis participates in the pathogenesis of recurrent miscarriage by inhibiting trophoblast migration and invasion.
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