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  • KD/KO Validated

USP25 Polyclonal antibody

USP25 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12199-1-AP

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Synonyms

Deubiquitinating enzyme 25, Ubiquitin thiolesterase 25, USP on chromosome 21, USP21, USP25



经过测试的应用

Positive WB detected inBxPC-3 cells, mouse cerebellum tissue, K-562 cells
Positive IP detected inRAW 264.7 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12199-1-AP targets USP25 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen USP25 fusion protein Ag2837 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin specific peptidase 25
Synonyms Deubiquitinating enzyme 25, Ubiquitin thiolesterase 25, USP on chromosome 21, USP21, USP25
Calculated Molecular Weight 125 kDa
Observed Molecular Weight 125 kDa
GenBank Accession NumberBC015930
Gene Symbol USP25
Gene ID (NCBI) 29761
RRIDAB_2212771
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHP3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ubiquitin carboxyl-terminal hydrolase 25, encoded by USP25 gene, has three isoforms by alternative splicing. Isoform USP25a is found in most adult and fetal tissues; Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific, and is upregulated during myogenesis, therefore, may have a role in the regulation of muscular differentiation and function. USP25, as deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates.

实验方案

Product Specific Protocols
WB protocol for USP25 antibody 12199-1-APDownload protocol
IHC protocol for USP25 antibody 12199-1-APDownload protocol
IP protocol for USP25 antibody 12199-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

An Interaction Landscape of Ubiquitin Signaling.

Authors - Xiaofei Zhang
humanWB

Cell Death Dis

Suppression of USP7 induces BCR-ABL degradation and chronic myelogenous leukemia cell apoptosis.

Authors - Shuoyi Jiang
mouseWB

Biochem Pharmacol

Inhibition of deubiquitinase USP28 attenuates cyst growth in autosomal dominant polycystic kidney disease

Authors - Ying Ren
humanWB

Nucleic Acids Res

The dimeric deubiquitinase USP28 integrates 53BP1 and MYC functions to limit DNA damage

Authors - Chao Jin
mouseWB

Ren Fail

USP25 attenuates anti-GBM nephritis in mice by negative feedback regulation of Th17 cell differentiation

Authors - Ranran Xu
  • KO Validated
humanIHC

J Cell Physiol

The miR-27a-3p/USP25 axis participates in the pathogenesis of recurrent miscarriage by inhibiting trophoblast migration and invasion.

Authors - Jinli Ding
  • KD Validated