验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, mouse cerebellum tissue, K-562 cells |
| Positive IP detected in | RAW 264.7 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
12199-1-AP targets USP25 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | USP25 fusion protein Ag2837 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin specific peptidase 25 |
| 别名 | Deubiquitinating enzyme 25, Ubiquitin thiolesterase 25, USP on chromosome 21, USP21, USP25 |
| 计算分子量 | 125 kDa |
| 观测分子量 | 125 kDa |
| GenBank蛋白编号 | BC015930 |
| 基因名称 | USP25 |
| Gene ID (NCBI) | 29761 |
| RRID | AB_2212771 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UHP3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ubiquitin carboxyl-terminal hydrolase 25, encoded by USP25 gene, has three isoforms by alternative splicing. Isoform USP25a is found in most adult and fetal tissues; Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific, and is upregulated during myogenesis, therefore, may have a role in the regulation of muscular differentiation and function. USP25, as deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USP25 antibody 12199-1-AP | Download protocol |
| IHC protocol for USP25 antibody 12199-1-AP | Download protocol |
| IP protocol for USP25 antibody 12199-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis Suppression of USP7 induces BCR-ABL degradation and chronic myelogenous leukemia cell apoptosis. | ||
Biochem Pharmacol Inhibition of deubiquitinase USP28 attenuates cyst growth in autosomal dominant polycystic kidney disease | ||
Nucleic Acids Res The dimeric deubiquitinase USP28 integrates 53BP1 and MYC functions to limit DNA damage | ||
Ren Fail USP25 attenuates anti-GBM nephritis in mice by negative feedback regulation of Th17 cell differentiation
| ||
J Cell Physiol The miR-27a-3p/USP25 axis participates in the pathogenesis of recurrent miscarriage by inhibiting trophoblast migration and invasion.
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