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验证数据展示

  • WB analysis of HEK-293T using 28817-1-AP

    WB analysis of HEK-293T using 28817-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 28817-1-AP (USP25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 28817-1-AP

    IHC staining of human colon using 28817-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28817-1-AP (USP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 28817-1-AP

    IHC staining of human colon using 28817-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28817-1-AP (USP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 28817-1-AP

    IHC staining of human lung cancer using 28817-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28817-1-AP (USP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

USP25 Polyclonal antibody

USP25 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28817-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Synonyms

Deubiquitinating enzyme 25, Ubiquitin thiolesterase 25, USP on chromosome 21, USP21, USP25

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, PC-3 cells, RAW 264.7 cells
Positive IHC detected inhuman lung cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28817-1-AP targets USP25 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen USP25 fusion protein Ag30463 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin specific peptidase 25
Synonyms Deubiquitinating enzyme 25, Ubiquitin thiolesterase 25, USP on chromosome 21, USP21, USP25
Calculated Molecular Weight 125 kDa
Observed Molecular Weight122-138 kDa
GenBank Accession NumberBC015930
Gene Symbol USP25
Gene ID (NCBI) 29761
RRIDAB_2918207
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHP3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ubiquitin carboxyl-terminal hydrolase 25, encoded by USP25 gene, has three isoforms by alternative splicing. Isoform USP25a is found in most adult and fetal tissues; Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific, and is upregulated during myogenesis, therefore, may have a role in the regulation of muscular differentiation and function. USP25, as a deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates.

实验方案

Product Specific Protocols
WB protocol for USP25 antibody 28817-1-APDownload protocol
IHC protocol for USP25 antibody 28817-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols