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验证数据展示

  • WB analysis of HEK-293T using 28817-1-AP

    WB analysis of HEK-293T using 28817-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 28817-1-AP (USP25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 28817-1-AP

    IHC staining of human colon using 28817-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28817-1-AP (USP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 28817-1-AP

    IHC staining of human colon using 28817-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28817-1-AP (USP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 28817-1-AP

    IHC staining of human lung cancer using 28817-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28817-1-AP (USP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

USP25 Polyclonal antibody

USP25 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28817-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, ELISA

别名

Deubiquitinating enzyme 25, Ubiquitin thiolesterase 25, USP on chromosome 21, USP21, USP25

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, PC-3 cells, RAW 264.7 cells
Positive IHC detected inhuman lung cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28817-1-AP targets USP25 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 USP25 fusion protein Ag30463 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 25
别名 Deubiquitinating enzyme 25, Ubiquitin thiolesterase 25, USP on chromosome 21, USP21, USP25
计算分子量 125 kDa
观测分子量122-138 kDa
GenBank蛋白编号BC015930
基因名称 USP25
Gene ID (NCBI) 29761
RRIDAB_2918207
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHP3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ubiquitin carboxyl-terminal hydrolase 25, encoded by USP25 gene, has three isoforms by alternative splicing. Isoform USP25a is found in most adult and fetal tissues; Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific, and is upregulated during myogenesis, therefore, may have a role in the regulation of muscular differentiation and function. USP25, as a deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates.

实验方案

Product Specific Protocols
WB protocol for USP25 antibody 28817-1-APDownload protocol
IHC protocol for USP25 antibody 28817-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols