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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67769-1-Ig (same clone as 67769-1-PBS)

    WB analysis using 67769-1-Ig (same clone as 67769-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67769-1-Ig (USP25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67769-1-PBS in a different storage buffer formulation.

  • WB analysis using 67769-1-Ig (same clone as 67769-1-PBS)

    WB analysis using 67769-1-Ig (same clone as 67769-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67769-1-Ig (USP25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67769-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 67769-1-Ig (same clone as 67769-1-PBS)

    IHC staining of mouse skeletal muscle using 67769-1-Ig (same clone as 67769-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67769-1-Ig (USP25 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67769-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 67769-1-Ig (same clone as 67769-1-PBS)

    IHC staining of mouse skeletal muscle using 67769-1-Ig (same clone as 67769-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67769-1-Ig (USP25 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67769-1-PBS in a different storage buffer formulation.

USP25 Monoclonal antibody, PBS Only

USP25 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67769-1-PBS

产品说明书

CloneNo. 2B6A2

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

Ubiquitin thiolesterase 25, Ubiquitin thioesterase 25, Ubiquitin carboxyl-terminal hydrolase 25, EC:3.4.19.12, Deubiquitinating enzyme 25

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67769-1-PBS targets USP25 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 USP25 fusion protein Ag28067 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 25
别名 Ubiquitin thiolesterase 25, Ubiquitin thioesterase 25, Ubiquitin carboxyl-terminal hydrolase 25, EC:3.4.19.12, Deubiquitinating enzyme 25
计算分子量 125 kDa
观测分子量125 kDa
GenBank蛋白编号BC015930
基因名称 USP25
Gene ID (NCBI) 29761
RRIDAB_2918535
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UHP3
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ubiquitin carboxyl-terminal hydrolase 25, encoded by USP25 gene, has three isoforms by alternative splicing. Isoform USP25a is found in most adult and fetal tissues; Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific, and is upregulated during myogenesis, therefore, may have a role in the regulation of muscular differentiation and function. USP25, as deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates.