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USP25 Monoclonal antibody, PBS Only

USP25 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B6A2

Cat no : 67769-1-PBS

Print datasheet

Synonyms

Ubiquitin thiolesterase 25, Ubiquitin thioesterase 25, Ubiquitin carboxyl-terminal hydrolase 25, EC:3.4.19.12, Deubiquitinating enzyme 25

验证数据展示

  • WB analysis using 67769-1-Ig (same clone as 67769-1-PBS)

    WB analysis using 67769-1-Ig (same clone as 67769-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67769-1-Ig (USP25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67769-1-PBS in a different storage buffer formulation.

  • WB analysis using 67769-1-Ig (same clone as 67769-1-PBS)

    WB analysis using 67769-1-Ig (same clone as 67769-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67769-1-Ig (USP25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67769-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 67769-1-Ig (same clone as 67769-1-PBS)

    IHC staining of mouse skeletal muscle using 67769-1-Ig (same clone as 67769-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67769-1-Ig (USP25 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67769-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 67769-1-Ig (same clone as 67769-1-PBS)

    IHC staining of mouse skeletal muscle using 67769-1-Ig (same clone as 67769-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67769-1-Ig (USP25 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67769-1-PBS in a different storage buffer formulation.

产品信息

67769-1-PBS targets USP25 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen USP25 fusion protein Ag28067 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ubiquitin specific peptidase 25
Synonyms Ubiquitin thiolesterase 25, Ubiquitin thioesterase 25, Ubiquitin carboxyl-terminal hydrolase 25, EC:3.4.19.12, Deubiquitinating enzyme 25
Calculated Molecular Weight 125 kDa
Observed Molecular Weight125 kDa
GenBank Accession NumberBC015930
Gene Symbol USP25
Gene ID (NCBI) 29761
RRIDAB_2918535
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UHP3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ubiquitin carboxyl-terminal hydrolase 25, encoded by USP25 gene, has three isoforms by alternative splicing. Isoform USP25a is found in most adult and fetal tissues; Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific, and is upregulated during myogenesis, therefore, may have a role in the regulation of muscular differentiation and function. USP25, as deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates.