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  • Featured Product
  • KD/KO Validated

USP29 Polyclonal antibody

USP29 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 27522-1-AP

Print datasheet

Synonyms

Deubiquitinating enzyme 29, HOM TES 84/86, Ubiquitin thiolesterase 29, USP29

验证数据展示

  • WB analysis using 27522-1-AP

    WB analysis using 27522-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27522-1-AP (USP29 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 27522-1-AP

    IHC staining of mouse testis using 27522-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27522-1-AP (USP29 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 27522-1-AP

    IHC staining of mouse testis using 27522-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27522-1-AP (USP29 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27522-1-AP targets USP29 in WB, IHC, IP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IP
Tested Reactivity Human, Mouse, Rat
Cited Reactivitymouse
Immunogen USP29 fusion protein Ag23231 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin specific peptidase 29
Synonyms Deubiquitinating enzyme 29, HOM TES 84/86, Ubiquitin thiolesterase 29, USP29
Observed Molecular Weight 104 kDa
GenBank Accession NumberBC130394
Gene Symbol USP29
Gene ID (NCBI) 57663
RRIDAB_2880898
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HBJ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for USP29 antibody 27522-1-APDownload protocol
IHC protocol for USP29 antibody 27522-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Oxid Med Cell Longev

Ubiquitin-Specific Protease 29 Exacerbates Cerebral Ischemia-Reperfusion Injury in Mice.

Authors - Jia-Bao Hou
  • KO Validated
View Article
mouseWB,IP

Redox Biol

ALDH2 deficiency augments atherosclerosis through the USP14-cGAS-dependent polarization of proinflammatory macrophages

Authors - Haiying Rui
View Article
mouseIHC

Clin Mol Hepatol

USP29 alleviates the progression of MASLD by stabilizing ACSL5 through K48 deubiquitination

Authors - Sha Hu
View Article