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USP30 Polyclonal antibody

USP30 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15402-1-AP

Print datasheet

Synonyms

Ubiquitin-specific-processing protease 30, Ubiquitin thiolesterase 30, Ubiquitin thioesterase 30, Ubiquitin carboxyl-terminal hydrolase 30, EC:3.4.19.12

验证数据展示

  • WB analysis using 15402-1-AP

    WB analysis using 15402-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15402-1-AP (USP30 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 15402-1-AP

    IF Staining of A549 using 15402-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using 15402-1-AP (USP30 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver cancer using 15402-1-AP

    IHC staining of human liver cancer using 15402-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15402-1-AP (USP30 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 15402-1-AP

    IHC staining of human liver cancer using 15402-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15402-1-AP (USP30 antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human colon cancer using 15402-1-AP

    IHC staining of human colon cancer using 15402-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15402-1-AP (USP30 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 15402-1-AP

    IHC staining of human colon cancer using 15402-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15402-1-AP (USP30 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inDaudi cells, HepG2 cells, hTERT-RPE1 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15402-1-AP targets USP30 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen USP30 fusion protein Ag7620 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin specific peptidase 30
Synonyms Ubiquitin-specific-processing protease 30, Ubiquitin thiolesterase 30, Ubiquitin thioesterase 30, Ubiquitin carboxyl-terminal hydrolase 30, EC:3.4.19.12
Calculated Molecular Weight58.5 kDa
Observed Molecular Weight 59-67 kDa
GenBank Accession NumberBC004868
Gene Symbol USP30
Gene ID (NCBI) 84749
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ70CQ3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP30, a mitochondrion-localized deubiquitylase, regulates mitophagy. Mitochondrial damage stimulates parkin to assemble Lys 6, Lys 11 and Lys 63 chains on mitochondria, and that USP30 is a ubiquitin-specific deubiquitylase with a strong preference for cleaving Lys 6- and Lys 11-linked multimers. USP30 can be ubiquitinated by parkin (PARK2) at Lys-235 and Lys-289. USP30 inhibition is potentially beneficial for treating Parkinson disease by promoting mitochondrial clearance and quality control. USP30 was detected 67 kDa by ubiquitination (PMID: 34704599).

实验方案

Product Specific Protocols
WB protocol for USP30 antibody 15402-1-APDownload protocol
IHC protocol for USP30 antibody 15402-1-APDownload protocol
IF protocol for USP30 antibody 15402-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF

Biochem Biophys Res Commun

USP30 impairs mitochondrial quality control and aggravates oxidative damage after traumatic brain injury

Authors - Yang Wu
View Article