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验证数据展示

  • WB analysis using 83240-5-RR (same clone as 83240-5-PBS)

    WB analysis using 83240-5-RR (same clone as 83240-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83240-5-RR (USP30 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83240-5-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 83240-5-RR (same clone as 83240-5-PBS)

    WB analysis of HepG2 using 83240-5-RR (same clone as 83240-5-PBS)

    WB result of USP30 antibody (83240-5-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP30 transfected HepG2 cells. This data was developed using the same antibody clone with 83240-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83240-5-RR (same clone as 83240-5-PBS)

    FC experiment of HeLa using 83240-5-RR (same clone as 83240-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug USP30 Recombinant antibody (83240-5-RR, Clone:240111C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83240-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83240-5-RR

    Affinity and Kinetic Characterization of 83240-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83240-5-RR against Human USP30 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

USP30 Recombinant antibody, PBS Only

USP30 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83240-5-PBS

产品说明书

CloneNo. 240111C6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Ubiquitin thiolesterase 30, Ubiquitin thioesterase 30, Ubiquitin carboxyl-terminal hydrolase 30, EC:3.4.19.12, Deubiquitinating enzyme 30

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83240-5-PBS targets USP30 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 USP30 fusion protein Ag7620 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin specific peptidase 30
别名 Ubiquitin thiolesterase 30, Ubiquitin thioesterase 30, Ubiquitin carboxyl-terminal hydrolase 30, EC:3.4.19.12, Deubiquitinating enzyme 30
计算分子量58.5 kDa
观测分子量 59-67 kDa
GenBank蛋白编号BC004868
基因名称 USP30
Gene ID (NCBI) 84749
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ70CQ3
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP30, a mitochondrion-localized deubiquitylase, regulates mitophagy. Mitochondrial damage stimulates parkin to assemble Lys 6, Lys 11 and Lys 63 chains on mitochondria, and that USP30 is a ubiquitin-specific deubiquitylase with a strong preference for cleaving Lys 6- and Lys 11-linked multimers. USP30 can be ubiquitinated by parkin (PARK2) at Lys-235 and Lys-289. USP30 inhibition is potentially beneficial for treating Parkinson disease by promoting mitochondrial clearance and quality control. USP30 was detected 67 kDa by ubiquitination (PMID: 34704599).