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  • Featured Product
  • KD/KO Validated

USP30 Recombinant antibody, PBS Only

USP30 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240111C6

Cat no : 83240-5-PBS

Print datasheet

Synonyms

Ubiquitin thiolesterase 30, Ubiquitin thioesterase 30, Ubiquitin carboxyl-terminal hydrolase 30, EC:3.4.19.12, Deubiquitinating enzyme 30

验证数据展示

  • WB analysis using 83240-5-RR (same clone as 83240-5-PBS)

    WB analysis using 83240-5-RR (same clone as 83240-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83240-5-RR (USP30 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83240-5-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 83240-5-RR (same clone as 83240-5-PBS)

    WB analysis of HepG2 using 83240-5-RR (same clone as 83240-5-PBS)

    WB result of USP30 antibody (83240-5-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP30 transfected HepG2 cells. This data was developed using the same antibody clone with 83240-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83240-5-RR (same clone as 83240-5-PBS)

    FC experiment of HeLa using 83240-5-RR (same clone as 83240-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug USP30 Recombinant antibody (83240-5-RR, Clone:240111C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83240-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83240-5-RR

    Affinity and Kinetic Characterization of 83240-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83240-5-RR against Human USP30 were performed. The affinity constant is below 1 pM.

产品信息

83240-5-PBS targets USP30 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen USP30 fusion protein Ag7620 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ubiquitin specific peptidase 30
Synonyms Ubiquitin thiolesterase 30, Ubiquitin thioesterase 30, Ubiquitin carboxyl-terminal hydrolase 30, EC:3.4.19.12, Deubiquitinating enzyme 30
Calculated Molecular Weight58.5 kDa
Observed Molecular Weight 59-67 kDa
GenBank Accession NumberBC004868
Gene Symbol USP30
Gene ID (NCBI) 84749
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ70CQ3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP30, a mitochondrion-localized deubiquitylase, regulates mitophagy. Mitochondrial damage stimulates parkin to assemble Lys 6, Lys 11 and Lys 63 chains on mitochondria, and that USP30 is a ubiquitin-specific deubiquitylase with a strong preference for cleaving Lys 6- and Lys 11-linked multimers. USP30 can be ubiquitinated by parkin (PARK2) at Lys-235 and Lys-289. USP30 inhibition is potentially beneficial for treating Parkinson disease by promoting mitochondrial clearance and quality control. USP30 was detected 67 kDa by ubiquitination (PMID: 34704599).