USP30 Recombinant antibody, PBS Only

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

ELISA, WB

Conjugate

Unconjugated

CloneNo.

240111C6

Cat No : 83240-5-PBS

Synonyms

Ubiquitin thiolesterase 30, Ubiquitin thioesterase 30, Ubiquitin carboxyl-terminal hydrolase 30, EC:3.4.19.12, Deubiquitinating enzyme 30

WB analysis using 83240-5-RR (same clone as 83240-5-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 83240-5-RR (USP30 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83240-5-PBS in a different storage buffer formulation.
FC experiment of HeLa using 83240-5-RR (same clone as 83240-5-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.25 ug USP30 Recombinant antibody (83240-5-RR, Clone:240111C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83240-5-PBS in a different storage buffer formulation.
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1x10^6 HeLa cells were intracellularly stained with 0.25 ug USP30 Recombinant antibody (83240-5-RR, Clone:240111C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83240-5-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 83240-5-RR
Biolayer interferometry (BLl) kinetic assays of 83240-5-RR against Human USP30 were performed. The affinity constant is below 1 pM.

Size

The immunogen of 83240-5-PBS is USP30 Fusion Protein expressed in E. coli.

Tested Applications	ELISA, WB Application Description
Tested Reactivity	human
Immunogen	USP30 fusion protein Ag7620 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Full Name	ubiquitin specific peptidase 30
Synonyms	Ubiquitin thiolesterase 30, Ubiquitin thioesterase 30, Ubiquitin carboxyl-terminal hydrolase 30, EC:3.4.19.12, Deubiquitinating enzyme 30
Calculated Molecular Weight	58.5 kDa
GenBank Accession Number	BC004868
Gene Symbol	USP30
Gene ID (NCBI)	84749
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	Q70CQ3
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C