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验证数据展示

  • WB analysis of HEK-293 using 12060-2-AP

    WB analysis of HEK-293 using 12060-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12060-2-AP (USP33 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human medulloblastoma using 12060-2-AP

    IHC staining of human medulloblastoma using 12060-2-AP

    Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12060-2-AP (USP33 antibody) at dilution of 1:50 (under 10x lens).

USP33 Polyclonal antibody

USP33 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12060-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Ubiquitin-specific-processing protease 33, Ubiquitin thioesterase 33, Ubiquitin carboxyl-terminal hydrolase 33, KIAA1097, hVDU1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman medulloblastoma tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12060-2-AP targets USP33 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 USP33 fusion protein Ag2695 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 33
别名 Ubiquitin-specific-processing protease 33, Ubiquitin thioesterase 33, Ubiquitin carboxyl-terminal hydrolase 33, KIAA1097, hVDU1
计算分子量 942 aa, 107 kDa
观测分子量 107 kDa/ 103 kDa/ 93 kDa
GenBank蛋白编号BC016663
基因名称 USP33
Gene ID (NCBI) 23032
RRIDAB_2213235
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TEY7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP33(Ubiquitin carboxyl-terminal hydrolase 33) is also named as KIAA1097, VDU1 and belongs to the peptidase C19 family. This protein, originally identified as a von Hippel-Lindau tumor suppressor (VHL) protein-interacting deubiquitinating enzyme, binds to the Robo1 receptor, and that USP33 is required for Slit responsiveness in breast cancer cells(PMID:19706539). The localization of USP33 is broadly confined to the secretory pathway, with all variants localizing to endoplasmic reticulum-associated structures(PMID:21801292). It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for USP33 antibody 12060-2-APDownload protocol
IHC protocol for USP33 antibody 12060-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols