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USP33 Polyclonal antibody

USP33 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12060-2-AP

Print datasheet

Synonyms

Deubiquitinating enzyme 33, hVDU1, KIAA1097, Ubiquitin thiolesterase 33, USP33, VDU1

验证数据展示

  • WB analysis of HEK-293 using 12060-2-AP

    WB analysis of HEK-293 using 12060-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12060-2-AP (USP33 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human medulloblastoma using 12060-2-AP

    IHC staining of human medulloblastoma using 12060-2-AP

    Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12060-2-AP (USP33 antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman medulloblastoma tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12060-2-AP targets USP33 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen USP33 fusion protein Ag2695 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin specific peptidase 33
Synonyms Deubiquitinating enzyme 33, hVDU1, KIAA1097, Ubiquitin thiolesterase 33, USP33, VDU1
Calculated Molecular Weight 942 aa, 107 kDa
Observed Molecular Weight 107 kDa/ 103 kDa/ 93 kDa
GenBank Accession NumberBC016663
Gene Symbol USP33
Gene ID (NCBI) 23032
RRIDAB_2213235
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TEY7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP33(Ubiquitin carboxyl-terminal hydrolase 33) is also named as KIAA1097, VDU1 and belongs to the peptidase C19 family. This protein, originally identified as a von Hippel-Lindau tumor suppressor (VHL) protein-interacting deubiquitinating enzyme, binds to the Robo1 receptor, and that USP33 is required for Slit responsiveness in breast cancer cells(PMID:19706539). The localization of USP33 is broadly confined to the secretory pathway, with all variants localizing to endoplasmic reticulum-associated structures(PMID:21801292). It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for USP33 antibody 12060-2-APDownload protocol
IHC protocol for USP33 antibody 12060-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols