USP35 Polyclonal antibody
USP35 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, RAW 264.7 cells |
| Positive IHC detected in | human colon cancer tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24559-1-AP targets USP35 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, CoIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | USP35 fusion protein Ag20002 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ubiquitin specific peptidase 35 |
| Synonyms | USP34, Ubiquitin-specific-processing protease 35, Ubiquitin carboxyl-terminal hydrolase 35, KIAA1372, EC:3.4.19.12 |
| Calculated Molecular Weight | 1018 aa, 113 kDa |
| Observed Molecular Weight | 84 kDa |
| GenBank Accession Number | BC131489 |
| Gene Symbol | USP35 |
| Gene ID (NCBI) | 57558 |
| RRID | AB_2879608 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P2H5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
USP35 is a member of the peptidase C19 family. USP35 is involved in cell cycle regulation, cell fate, and cancer development. USP35 regulates cell mitosis by deubiquitinating Aurora B and prevents its degradation from APC/CDH. USP35 can also regulate MFN2 abundance and PARK2-mediated mitophagy. USP35 has 2 isoforms with the molecular mass of 84 and 113 kDa (PMID: 34618999).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USP35 antibody 24559-1-AP | Download protocol |
| IHC protocol for USP35 antibody 24559-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Commun Biol Overexpression of USP35 enhances the protective effect of hUC-MSCs and their extracellular vesicles in oxygen-glucose deprivation/reperfusion-induced SH-SY5Y cells via stabilizing FUNDC1
| ||
Cell Death Discov Deubiquitinase USP35 stabilizes BRPF1 to activate mevalonate (MVA) metabolism during prostate tumorigenesis | ||
Oncogenesis USP35 promotes cell proliferation and chemotherapeutic resistance through stabilizing FUCA1 in colorectal cancer | ||
Biochem Biophys Res Commun Regulation of survivin protein stability by USP35 is evolutionarily conserved |
