验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse spleen tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18465-1-AP targets USP37 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | USP37 fusion protein Ag12513 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin specific peptidase 37 |
| 别名 | Deubiquitinating enzyme 37, KIAA1594, Ubiquitin thiolesterase 37, USP37 |
| 计算分子量 | 885 aa, 100 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC112901 |
| 基因名称 | USP37 |
| Gene ID (NCBI) | 57695 |
| RRID | AB_10598483 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86T82 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
USP37 (ubiquitin specific peptidase 37), is a member of ubiquitin-specific processing proteases family. Human USP37, localized mainly in the cytoplasm, is composed of 979 amino acids harboring three ubiquitin-interacting motifs between the Cys box and His box of the primary sequence (PMID: 27045719, 24324262). High levels of USP37 gene expression in lung cancer promoted cell viability as well as the Warburg effect via deubiquitination and stabilization of pluripotent factor c-Myc protein (PMID: 25284584). Moreover, USP37 expression was upregulated in breast cancer tissues compared with surrounding tissues and its overexpression was significantly correlated with increased rates of mortality. USP37 may represent a novel molecular target for breast cancer treatment (PMID: 30482232, 27045719).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USP37 antibody 18465-1-AP | Download protocol |
| IHC protocol for USP37 antibody 18465-1-AP | Download protocol |
| IP protocol for USP37 antibody 18465-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res USP37 regulates DNA damage response through stabilizing and deubiquitinating BLM.
| ||
Proc Natl Acad Sci U S A CYLD regulates spindle orientation by stabilizing astral microtubules and promoting dishevelled-NuMA-dynein/dynactin complex formation. | ||
Theranostics PLAGL2 promotes the proliferation and migration of gastric cancer cells via USP37-mediated deubiquitination of Snail1.
| ||
Front Immunol Specific Deubiquitinating Enzymes Promote Host Restriction Factors Against HIV/SIV Viruses.
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