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USP4 Recombinant antibody, PBS Only (Capture)

USP4 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240797B11

Cat no : 83734-2-PBS

Print datasheet

Synonyms

Unph, UNP, Ubiquitin-specific-processing protease 4, Ubiquitin carboxyl-terminal hydrolase 4, EC:3.4.19.12

验证数据展示

  • WB analysis using 83734-2-RR (same clone as 83734-2-PBS)

    WB analysis using 83734-2-RR (same clone as 83734-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83734-2-RR (USP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83734-2-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 83734-2-RR (same clone as 83734-2-PBS)

    FC experiment of U-251 using 83734-2-RR (same clone as 83734-2-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug USP4 Recombinant antibody (83734-2-RR, Clone:240797B11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83734-2-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83734-2-RR (same clone as 83734-2-PBS)

    IHC staining of human ovary cancer using 83734-2-RR (same clone as 83734-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83734-2-RR (USP4 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83734-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00717-1

    Cytometric bead array standard curve of MP00717-1

    Cytometric bead array standard curve of MP00717-1, USP4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83734-2-PBS. Detection antibody: 83734-4-PBS. Standard: Ag28297. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83734-2-RR

    Affinity and Kinetic Characterization of 83734-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83734-2-RR against Human USP4 were performed. The affinity constant is 0.598 nM.

产品信息

83734-2-PBS targets USP4 as part of a matched antibody pair:

MP00717-1: 83734-2-PBS capture and 83734-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen USP4 fusion protein Ag28297 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ubiquitin specific peptidase 4 (proto-oncogene)
Synonyms Unph, UNP, Ubiquitin-specific-processing protease 4, Ubiquitin carboxyl-terminal hydrolase 4, EC:3.4.19.12
Calculated Molecular Weight 963 aa, 109 kDa
Observed Molecular Weight 109 kDa
GenBank Accession NumberBC125131
Gene Symbol USP4
Gene ID (NCBI) 7375
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13107
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP4, also known as UNP and UNPH, belongs to the peptidase C19 family and USP4 subfamily. USP4 is a deubiquitinating enzyme that links to mitogen-activated protein kinase signaling, pre-mRNA splicing, and control of p53 stability (PMID: 26455393). USP4 has 3 isoforms with the molecular mass of 36, 104 and 109 kDa. Recently, it has been reported that USP4 is a critical factor in promoting lung cancer stemness and potentially useful lung cancer prognosis marker (PMID: 32549341).