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验证数据展示

  • WB analysis using 83734-2-RR (same clone as 83734-2-PBS)

    WB analysis using 83734-2-RR (same clone as 83734-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83734-2-RR (USP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83734-2-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 83734-2-RR (same clone as 83734-2-PBS)

    FC experiment of U-251 using 83734-2-RR (same clone as 83734-2-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug USP4 Recombinant antibody (83734-2-RR, Clone:240797B11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83734-2-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83734-2-RR (same clone as 83734-2-PBS)

    IHC staining of human ovary cancer using 83734-2-RR (same clone as 83734-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83734-2-RR (USP4 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83734-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00717-1

    Cytometric bead array standard curve of MP00717-1

    Cytometric bead array standard curve of MP00717-1, USP4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83734-2-PBS. Detection antibody: 83734-4-PBS. Standard: Ag28297. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83734-2-PBS

    Affinity and Kinetic Characterization of 83734-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83734-2-PBS against Human USP4 was performed. The affinity constant is 3.51 nM.

  • Affinity and Kinetic Characterization of 83734-2-RR

    Affinity and Kinetic Characterization of 83734-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83734-2-RR against Human USP4 were performed. The affinity constant is 0.598 nM.

USP4 Recombinant antibody, PBS Only (Capture)

USP4 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83734-2-PBS

产品说明书

CloneNo. 240797B11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

Unph, UNP, Ubiquitin-specific-processing protease 4, Ubiquitin carboxyl-terminal hydrolase 4, EC:3.4.19.12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83734-2-PBS targets USP4 as part of a matched antibody pair:

MP00717-1: 83734-2-PBS capture and 83734-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 USP4 fusion protein Ag28297 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin specific peptidase 4 (proto-oncogene)
别名 Unph, UNP, Ubiquitin-specific-processing protease 4, Ubiquitin carboxyl-terminal hydrolase 4, EC:3.4.19.12
计算分子量 963 aa, 109 kDa
观测分子量 109 kDa
GenBank蛋白编号BC125131
基因名称 USP4
Gene ID (NCBI) 7375
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13107
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP4, also known as UNP and UNPH, belongs to the peptidase C19 family and USP4 subfamily. USP4 is a deubiquitinating enzyme that links to mitogen-activated protein kinase signaling, pre-mRNA splicing, and control of p53 stability (PMID: 26455393). USP4 has 3 isoforms with the molecular mass of 36, 104 and 109 kDa. Recently, it has been reported that USP4 is a critical factor in promoting lung cancer stemness and potentially useful lung cancer prognosis marker (PMID: 32549341).