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验证数据展示

  • WB analysis using 27217-1-AP

    WB analysis using 27217-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27217-1-AP (USP40 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 27217-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 27217-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27217-1-AP (USP40 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 27217-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 27217-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27217-1-AP (USP40 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

USP40 Polyclonal antibody

USP40 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27217-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

USP 40, Ubiquitin-specific-processing protease 40, Ubiquitin thioesterase 40, Ubiquitin carboxyl-terminal hydrolase 40, EC:3.4.19.12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27217-1-AP targets USP40 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 USP40 fusion protein Ag25976 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 40
别名 USP 40, Ubiquitin-specific-processing protease 40, Ubiquitin thioesterase 40, Ubiquitin carboxyl-terminal hydrolase 40, EC:3.4.19.12
观测分子量140 kDa
GenBank蛋白编号BC013305
基因名称 USP40
Gene ID (NCBI) 55230
RRIDAB_3085938
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NVE5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP40 (ubiquitin specific peptidase 40), also known as ubiquitin thioesterase 40, deubiquitinating enzyme 40 or ubiquitin carboxyl-terminal hydrolase 40, is a 1235 amino acid protein that belongs to a large family of cysteine proteases that function as deubiquitinating enzymes. USP40 transcript is ubiquitously expressed but abundant in the kidney, heart, spleen, liver, and testis. Within the kidney, USP40 is expressed in glomerular endothelial cells during development (PMID:28148530).

实验方案

Product Specific Protocols
WB protocol for USP40 antibody 27217-1-APDownload protocol
IHC protocol for USP40 antibody 27217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols