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USP40 Polyclonal antibody

USP40 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27217-1-AP

Print datasheet

Synonyms

USP 40, Ubiquitin-specific-processing protease 40, Ubiquitin thioesterase 40, Ubiquitin carboxyl-terminal hydrolase 40, EC:3.4.19.12

验证数据展示

  • WB analysis using 27217-1-AP

    WB analysis using 27217-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27217-1-AP (USP40 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 27217-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 27217-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27217-1-AP (USP40 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 27217-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 27217-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27217-1-AP (USP40 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27217-1-AP targets USP40 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen USP40 fusion protein Ag25976 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin specific peptidase 40
Synonyms USP 40, Ubiquitin-specific-processing protease 40, Ubiquitin thioesterase 40, Ubiquitin carboxyl-terminal hydrolase 40, EC:3.4.19.12
Observed Molecular Weight140 kDa
GenBank Accession NumberBC013305
Gene Symbol USP40
Gene ID (NCBI) 55230
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVE5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP40 (ubiquitin specific peptidase 40), also known as ubiquitin thioesterase 40, deubiquitinating enzyme 40 or ubiquitin carboxyl-terminal hydrolase 40, is a 1235 amino acid protein that belongs to a large family of cysteine proteases that function as deubiquitinating enzymes. USP40 transcript is ubiquitously expressed but abundant in the kidney, heart, spleen, liver, and testis. Within the kidney, USP40 is expressed in glomerular endothelial cells during development (PMID:28148530).

实验方案

Product Specific Protocols
WB protocol for USP40 antibody 27217-1-APDownload protocol
IHC protocol for USP40 antibody 27217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols