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USP42 Polyclonal antibody

USP42 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18811-1-AP

Print datasheet

Synonyms

USP42

验证数据展示

  • WB analysis of mouse brain using 18811-1-AP

    WB analysis of mouse brain using 18811-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18811-1-AP (USP42 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 18811-1-AP

    IHC staining of mouse brain using 18811-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18811-1-AP (USP42 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

18811-1-AP targets USP42 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin specific peptidase 42
Synonyms USP42
Calculated Molecular Weight 145 kDa
Observed Molecular Weight 146 kDa
GenBank Accession NumberBC136656
Gene Symbol USP42
Gene ID (NCBI) 84132
RRIDAB_10603481
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H9J4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP42(ubiquitin carboxyl-terminal hydrolase 42) is also named as deubiquitinating enzyme 42 and belongs to the peptidase C19 family. It consists of 1,324 amino acids with a predicted molecular weight of 146 kDa and contains the conserved Cys, Asp (I), His and Asn/Asp (II) domains defined as one of characteristics for deubiquitinating enzymes, which may play an important role in mouse embryogenesis and spermatogenesis(PMID:16904385). This protein has 2 isoforms produced by alternative splicing. This antibody is specific to USP42.

实验方案

Product Specific Protocols
WB protocol for USP42 antibody 18811-1-APDownload protocol
IHC protocol for USP42 antibody 18811-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Differ

USP42 drives nuclear speckle mRNA splicing via directing dynamic phase separation to promote tumorigenesis.

Authors - Shuyan Liu
View Article