验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, mouse testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15521-1-AP targets USP44 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | USP44 fusion protein Ag7888 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin specific peptidase 44 |
| 别名 | Deubiquitinating enzyme 44, Ubiquitin thiolesterase 44, USP44 |
| 计算分子量 | 81 kDa |
| 观测分子量 | 81 kDa |
| GenBank蛋白编号 | BC030704 |
| 基因名称 | USP44 |
| Gene ID (NCBI) | 84101 |
| RRID | AB_2213688 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H0E7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
USP44(Ubiquitin carboxyl-terminal hydrolase 44) belongs to a large family of cysteine proteases that function as deubiquitinating enzymes. It has a ZnF-UBP (zinc-finger ubiquitin-specific protease) domain and conserved cysteine, histidine and asparagine/aspartic acid residues characteristic of deubiquitinating enzymes. And USP44 can cleave ubiquitin from ubiquitinated substrates both in vitro and in vivo. The expression of endogenous mouse or human USP44 is very low. A localization study of USP44 showed its predominant expression in the nucleus(PMID:20402667).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USP44 antibody 15521-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest USP44 regulates centrosome positioning to prevent aneuploidy and suppress tumorigenesis. | ||
Nucleic Acids Res USP11 acts as a histone deubiquitinase functioning in chromatin reorganization during DNA repair. |
