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  • Featured Product
  • KD/KO Validated

USP5 Polyclonal antibody, PBS Only

USP5 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 15158-1-PBS

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Synonyms

验证数据展示

  • WB analysis of mouse lung using 15158-1-AP (same clone as 15158-1-PBS)

    WB analysis of mouse lung using 15158-1-AP (same clone as 15158-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 15158-1-AP (same clone as 15158-1-PBS)

    WB analysis of A549 using 15158-1-AP (same clone as 15158-1-PBS)

    WB result of USP5 antibody (15158-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP5 transfected A549 cells. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 15158-1-AP (same clone as 15158-1-PBS)

    IP experiment of mouse brain using 15158-1-AP (same clone as 15158-1-PBS)

    IP result of anti-USP5 (IP:15158-1-AP, 4ug; Detection:15158-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

产品信息

15158-1-PBS targets USP5 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen USP5 fusion protein Ag7252 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin specific peptidase 5 (isopeptidase T)
Synonyms
Calculated Molecular Weight 96 kDa
Observed Molecular Weight 95-105 kDa
GenBank Accession NumberBC005139
Gene Symbol USP5
Gene ID (NCBI) 8078
RRIDAB_2213856
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP45974
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers.