Various lysates were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

Jurkat cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

WB result of USP5 antibody (66213-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP5 transfected A549 cells. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed A549 cells using USP5 antibody (66213-1-Ig, Clone: 3F12C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.