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验证数据展示

  • WB analysis using 30440-1-AP

    WB analysis using 30440-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30440-1-AP (USP6NL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 30440-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 30440-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30440-1-AP (USP6NL antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 30440-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 30440-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30440-1-AP (USP6NL antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 30440-1-AP

    WB analysis of HeLa using 30440-1-AP

    WB result of USP6NL antibody (30440-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP6NL transfected HeLa cells.

  • Featured Product
  • KD/KO Validated

USP6NL Polyclonal antibody

USP6NL Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30440-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

USP6 N-terminal-like protein, Related to the N-terminus of tre, KIAA0019

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHT-29 cells, HeLa cells, SW480 cells, Jurkat cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30440-1-AP targets USP6NL in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen USP6NL fusion protein Ag32603 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name USP6 N-terminal like
Synonyms USP6 N-terminal-like protein, Related to the N-terminus of tre, KIAA0019
Calculated Molecular Weight 828 aa, 94 kDa
Observed Molecular Weight94 kDa
GenBank Accession NumberBC042943
Gene Symbol USP6NL
Gene ID (NCBI) 9712
RRIDAB_3086315
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92738
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP6NL, also named RN-tre, is a GTPase-activating protein (GAP), which stimulates GTP hydrolysis on various members of the Rab family, thereby inhibiting endocytosis of PM receptors and retrograde Golgi transport. It is highly expressed in many cancer types and may promote the growth and proliferation of cancer cells.

实验方案

Product Specific Protocols
WB protocol for USP6NL antibody 30440-1-APDownload protocol
IHC protocol for USP6NL antibody 30440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols