• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 30440-1-AP

    WB analysis using 30440-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30440-1-AP (USP6NL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 30440-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 30440-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30440-1-AP (USP6NL antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 30440-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 30440-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30440-1-AP (USP6NL antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 30440-1-AP

    WB analysis of HeLa using 30440-1-AP

    WB result of USP6NL antibody (30440-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP6NL transfected HeLa cells.

  • Featured Product
  • KD/KO验证

USP6NL Polyclonal antibody

USP6NL Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30440-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

USP6 N-terminal-like protein, Related to the N-terminus of tre, KIAA0019

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHT-29 cells, HeLa cells, SW480 cells, Jurkat cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30440-1-AP targets USP6NL in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 USP6NL fusion protein Ag32603 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 USP6 N-terminal like
别名 USP6 N-terminal-like protein, Related to the N-terminus of tre, KIAA0019
计算分子量 828 aa, 94 kDa
观测分子量94 kDa
GenBank蛋白编号BC042943
基因名称 USP6NL
Gene ID (NCBI) 9712
RRIDAB_3086315
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92738
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP6NL, also named RN-tre, is a GTPase-activating protein (GAP), which stimulates GTP hydrolysis on various members of the Rab family, thereby inhibiting endocytosis of PM receptors and retrograde Golgi transport. It is highly expressed in many cancer types and may promote the growth and proliferation of cancer cells.

实验方案

Product Specific Protocols
WB protocol for USP6NL antibody 30440-1-APDownload protocol
IHC protocol for USP6NL antibody 30440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols