验证数据展示
经过测试的应用
Positive WB detected in | HT-29 cells, HeLa cells, SW480 cells, Jurkat cells |
Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30440-1-AP targets USP6NL in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | USP6NL fusion protein Ag32603 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | USP6 N-terminal like |
Synonyms | USP6 N-terminal-like protein, Related to the N-terminus of tre, KIAA0019 |
Calculated Molecular Weight | 828 aa, 94 kDa |
Observed Molecular Weight | 94 kDa |
GenBank Accession Number | BC042943 |
Gene Symbol | USP6NL |
Gene ID (NCBI) | 9712 |
RRID | AB_3086315 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q92738 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
USP6NL, also named RN-tre, is a GTPase-activating protein (GAP), which stimulates GTP hydrolysis on various members of the Rab family, thereby inhibiting endocytosis of PM receptors and retrograde Golgi transport. It is highly expressed in many cancer types and may promote the growth and proliferation of cancer cells.
实验方案
Product Specific Protocols | |
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WB protocol for USP6NL antibody 30440-1-AP | Download protocol |
IHC protocol for USP6NL antibody 30440-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |