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USP8 Monoclonal antibody, PBS Only

USP8 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E12E3

Cat No : 67321-1-PBS

Print datasheet

Synonyms

Ubiquitin carboxyl-terminal hydrolase 8, KIAA0055, HumORF8, hUBPy, EC:3.4.19.12

验证数据展示

  • WB analysis using 67321-1-Ig (same clone as 67321-1-PBS)

    WB analysis using 67321-1-Ig (same clone as 67321-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67321-1-Ig (USP8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67321-1-Ig (same clone as 67321-1-PBS)

    IHC staining of human breast cancer using 67321-1-Ig (same clone as 67321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67321-1-Ig (USP8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67321-1-Ig (same clone as 67321-1-PBS)

    IHC staining of human breast cancer using 67321-1-Ig (same clone as 67321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67321-1-Ig (USP8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

  • FC experiment of Hela using 67321-1-Ig (same clone as 67321-1-PBS)

    FC experiment of Hela using 67321-1-Ig (same clone as 67321-1-PBS)

    1X10^6 Hela cells were intracellularly stained with 0.4 ug Anti-Human USP8 (67321-1-Ig, Clone:1E12E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 67321-1-Ig (same clone as 67321-1-PBS)

    WB analysis of NIH/3T3 using 67321-1-Ig (same clone as 67321-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67321-1-Ig (USP8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67321-1-Ig (same clone as 67321-1-PBS)

    IF Staining of HeLa using 67321-1-Ig (same clone as 67321-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP8 antibody (67321-1-Ig, Clone: 1E12E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67321-1-Ig (same clone as 67321-1-PBS)

    FC experiment of HeLa using 67321-1-Ig (same clone as 67321-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human USP8 (67321-1-Ig, Clone:1E12E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

产品信息

67321-1-PBS targets USP8 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with mouse, human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity mouse, human
Immunogen USP8 fusion protein Ag27104 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin specific peptidase 8
Synonyms Ubiquitin carboxyl-terminal hydrolase 8, KIAA0055, HumORF8, hUBPy, EC:3.4.19.12
Calculated Molecular Weight 1118 aa, 128 kDa
Observed Molecular Weight 128 kDa
GenBank Accession NumberBC110590
Gene Symbol USP8
Gene ID (NCBI) 9101
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP40818
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C