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USP8 Recombinant antibody

USP8 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240016D3

Cat No : 83172-2-RR

Print datasheet

Synonyms

Ubiquitin carboxyl-terminal hydrolase 8, KIAA0055, HumORF8, hUBPy, EC:3.4.19.12

验证数据展示

  • WB analysis using 83172-2-RR

    WB analysis using 83172-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83172-2-RR (USP8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 83172-2-RR

    IF Staining of U2OS using 83172-2-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of U2OS using 83172-2-RR

    IF Staining of U2OS using 83172-2-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of A549 using 83172-2-RR

    FC experiment of A549 using 83172-2-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug USP8 Recombinant antibody (83172-2-RR, Clone:240016D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83172-2-RR

    Affinity and Kinetic Characterization of 83172-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83172-2-RR against Human USP8 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHCT 116 cells, K-562 cells, A549 cells, HepG2 cells, HEK-293 cells, HeLa cells, MCF-7 cells
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83172-2-RR targets USP8 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen USP8 fusion protein Ag27143 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ubiquitin specific peptidase 8
Synonyms Ubiquitin carboxyl-terminal hydrolase 8, KIAA0055, HumORF8, hUBPy, EC:3.4.19.12
Calculated Molecular Weight 1118 aa, 128 kDa
GenBank Accession NumberBC110590
Gene Symbol USP8
Gene ID (NCBI) 9101
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP40818
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP8 is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage.

实验方案

Product Specific Protocols
WB protocol for USP8 antibody 83172-2-RRDownload protocol
IF protocol for USP8 antibody 83172-2-RRDownload protocol
FC protocol for USP8 antibody 83172-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols