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验证数据展示

  • WB analysis of HEK-293 using 81892-1-RR (same clone as 81892-1-PBS)

    WB analysis of HEK-293 using 81892-1-RR (same clone as 81892-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 81892-1-RR (USP9X antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81892-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 81892-1-RR (same clone as 81892-1-PBS)

    WB analysis of HEK-293 using 81892-1-RR (same clone as 81892-1-PBS)

    WB result of USP9X antibody (81892-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP9X transfected HEK-293 cells. This data was developed using the same antibody clone with 81892-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 81892-1-RR (same clone as 81892-1-PBS)

    IF Staining of HeLa using 81892-1-RR (same clone as 81892-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using USP9X antibody (81892-1-RR, Clone: 1B23 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81892-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 81892-1-RR (same clone as 81892-1-PBS)

    IP experiment of HEK-293 using 81892-1-RR (same clone as 81892-1-PBS)

    IP result of anti-USP9X (IP:81892-1-RR, 4ug; Detection:81892-1-RR 1:3000) with HEK-293 cells lysate 1640 ug. This data was developed using the same antibody clone with 81892-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 81892-1-RR (same clone as 81892-1-PBS)

    IF Staining of mouse testis using 81892-1-RR (same clone as 81892-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using USP9X antibody (81892-1-RR, Clone: 1B23 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81892-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

USP9X Recombinant antibody, PBS Only

USP9X Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 81892-1-PBS

产品说明书

CloneNo. 1B23

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

FAM, FAF, EC:3.4.19.12, DFFRX, Deubiquitinating enzyme FAF-X

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

81892-1-PBS targets USP9X in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin specific peptidase 9, X-linked
别名 FAM, FAF, EC:3.4.19.12, DFFRX, Deubiquitinating enzyme FAF-X
计算分子量 292 kDa
观测分子量260-290 kDa
GenBank蛋白编号NM_001039590
基因名称 USP9X
Gene ID (NCBI) 8239
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ93008
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP9X, also named as DFFRX, FAM and USP9, belongs to the peptidase C19 family. It may function as a ubiquitin-protein or polyubiquitin hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. It plays an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. USP9X is an essential component of TGF-beta/BMP signaling cascade. Deubiqitination of SMAD4 by USP9X restores its competence to mediate TGF-beta signaling. USP9X regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. The antibody is specific to USP9X. It has no cross reaction to USP9Y.