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1A6/DRIM Polyclonal antibody

1A6/DRIM Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18830-1-AP

Print datasheet

Synonyms

DRIM, NNP73, Novel nucleolar protein 73, Protein Key 1A6, UTP20

验证数据展示

  • WB analysis of HeLa using 18830-1-AP

    WB analysis of HeLa using 18830-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18830-1-AP (1A6/DRIM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 18830-1-AP

    IF Staining of HeLa using 18830-1-AP

    Immunofluorescent analysis of Hela cells, using UTP20 antibody 18830-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of HeLa using 18830-1-AP

    IP experiment of HeLa using 18830-1-AP

    IP result of anti-1A6/DRIM (IP:18830-1-AP, 4ug; Detection:18830-1-AP 1:800) with HeLa cells lysate 4000ug.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, mouse ovary tissue
Positive IP detected inHeLa cells
Positive IF/ICC detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18830-1-AP targets 1A6/DRIM in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name UTP20, small subunit (SSU) processome component, homolog (yeast)
Synonyms DRIM, NNP73, Novel nucleolar protein 73, Protein Key 1A6, UTP20
Calculated Molecular Weight 318 kDa
Observed Molecular Weight 310 kDa
GenBank Accession NumberNM_014503
Gene Symbol UTP20
Gene ID (NCBI) 27340
RRIDAB_10597541
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75691
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UTP20, also named as DRIM, interacts with KIAA0649.

实验方案

Product Specific Protocols
WB protocol for 1A6/DRIM antibody 18830-1-APDownload protocol
IF protocol for 1A6/DRIM antibody 18830-1-APDownload protocol
IP protocol for 1A6/DRIM antibody 18830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols