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UTRN Polyclonal antibody

UTRN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27948-1-AP

Print datasheet

Synonyms

DMDL, DRP, DRP 1, DRP1, Dystrophin related protein 1, UTRN, utrophin

验证数据展示

  • WB analysis using 27948-1-AP

    WB analysis using 27948-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27948-1-AP (UTRN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 27948-1-AP

    IHC staining of human colon using 27948-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27948-1-AP (UTRN antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Utrophin antibody included
  • IHC staining of human colon using 27948-1-AP

    IHC staining of human colon using 27948-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27948-1-AP (UTRN antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Utrophin antibody included

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Utrophin Ready-To-Use IHC Kit. Utrophin primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27948-1-AP targets UTRN in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen UTRN fusion protein Ag27497 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name utrophin
Synonyms DMDL, DRP, DRP 1, DRP1, Dystrophin related protein 1, UTRN, utrophin
Calculated Molecular Weight 395 kDa
Observed Molecular Weight 394 kDa, 70 kDa
GenBank Accession NumberNM_007124
Gene Symbol UTRN
Gene ID (NCBI) 7402
RRIDAB_2881018
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP46939
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Utrophin is a large protein (395 kDa) which is widely expressed and most abundant in several non-skeletal muscle tissues, for example lung, intestine, embryonic neural tube, sensory ganglia, tendons and ossifying cartilages. Two alternate intronic transcripts of utrophin have been described: Up71 and Up140.

实验方案

Product Specific Protocols
WB protocol for UTRN antibody 27948-1-APDownload protocol
IHC protocol for UTRN antibody 27948-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols