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UTX Polyclonal antibody

UTX Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 23984-1-AP

Print datasheet

Synonyms

Histone demethylase UTX, KDM6A, Lysine specific demethylase 6A, UTX

验证数据展示

  • WB analysis of COLO 320 using 23984-1-AP

    WB analysis of COLO 320 using 23984-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 23984-1-AP (UTX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 23984-1-AP

    WB analysis of HepG2 using 23984-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23984-1-AP (UTX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis using 23984-1-AP

    WB analysis using 23984-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23984-1-AP (UTX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inCOLO 320 cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

23984-1-AP targets UTX in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen UTX fusion protein Ag20018 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitously transcribed tetratricopeptide repeat, X chromosome
Synonyms Histone demethylase UTX, KDM6A, Lysine specific demethylase 6A, UTX
Calculated Molecular Weight 1401 aa, 154 kDa
Observed Molecular Weight154 kDa
GenBank Accession NumberBC093868
Gene Symbol UTX
Gene ID (NCBI) 7403
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15550
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KDM6A, also named UTX, mediates the removal of repressive trimethylation of histone H3, it plays a central role in the regulation of posterior development, by regulating HOX gene expression (PubMed:17851529).

实验方案

Product Specific Protocols
WB protocol for UTX antibody 23984-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Eur J Med Res

Inactivation of KDM6A promotes the progression of colorectal cancer by enhancing the glycolysis

Authors - Dexiang Zhang
View Article