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验证数据展示

  • WB analysis using 26201-1-AP

    WB analysis using 26201-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26201-1-AP (Unc119b antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of MDCK using 26201-1-AP

    IF Staining of MDCK using 26201-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 26201-1-AP (Unc119b antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Unc119b Polyclonal antibody

Unc119b Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26201-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, Canine

应用

WB, IF/ICC, ELISA

别名

AA407659, AI414892, unc 119 homolog B (C. elegans), Unc119b

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, MDCK cells, mouse kidney tissue, HEK-293 cells, HeLa cells
Positive IF/ICC detected inMDCK cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26201-1-AP targets Unc119b in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, Canine samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, Canine
免疫原 Unc119b fusion protein Ag24599 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 unc-119 homolog B (C. elegans)
别名 AA407659, AI414892, unc 119 homolog B (C. elegans), Unc119b
计算分子量 28 kDa
观测分子量 28-31 kDa
GenBank蛋白编号BC008617
基因名称 Unc119b
Gene ID (NCBI) 106840
RRIDAB_2880422
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8C4B4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mouse Unc119b is related closely to Unc119, a homolog of C. elegans unc119 protein which was first discovered in C. elegans on the basis of a spontaneous mutation affecting locomotion, feeding behavior and chemosensation. Mouse Unc119b shares 90% sequence identity with human UNC119b protein. UNC119b is a myristoyl-binding protein that acts as a cargo adapter: specifically binds the myristoyl moiety of a subset of N-terminally myristoylated proteins and is required for their localization. Myristoyl-binding activity of UNC119b is required to localize NPHP3 to the primary cilium (PMID: 22085962).

实验方案

Product Specific Protocols
WB protocol for Unc119b antibody 26201-1-APDownload protocol
IF protocol for Unc119b antibody 26201-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols