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验证数据展示

  • WB analysis of rat lung using 26909-1-AP

    WB analysis of rat lung using 26909-1-AP

    rat lung tissue were subjected to SDS PAGE followed by western blot with 26909-1-AP (Uteroglobin Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 26909-1-AP

    IHC staining of human lung using 26909-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 26909-1-AP (Uteroglobin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Uteroglobin/SCGB1A1 antibody included
  • IHC staining of human lung using 26909-1-AP

    IHC staining of human lung using 26909-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 26909-1-AP (Uteroglobin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Uteroglobin/SCGB1A1 antibody included
  • IF Staining of A549 using 26909-1-AP

    IF Staining of A549 using 26909-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 26909-1-AP (Uteroglobin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of A549 using 26909-1-AP

    FC experiment of A549 using 26909-1-AP

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human Uteroglobin/CC10 (26909-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Uteroglobin/CC10 Polyclonal antibody

Uteroglobin/CC10 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 26909-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA and More (1)

Synonyms

SCGB1A1, Uteroglobin, Club cells 10 kDa secretory protein, Club cell phospholipid-binding protein, CCSP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inrat lung tissue
Positive IHC detected inhuman lung tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy Uteroglobin/SCGB1A1 Ready-To-Use IHC Kit. Uteroglobin/SCGB1A1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26909-1-AP targets Uteroglobin/CC10 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsIHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Uteroglobin/CC10 fusion protein Ag25530 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name secretoglobin, family 1A, member 1 (uteroglobin)
Synonyms SCGB1A1, Uteroglobin, Club cells 10 kDa secretory protein, Club cell phospholipid-binding protein, CCSP
Calculated Molecular Weight 10 kDa
Observed Molecular Weight 9 kDa
GenBank Accession NumberBC004481
Gene Symbol Uteroglobin
Gene ID (NCBI) 7356
RRIDAB_2880680
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11684
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Uteroglobin, also known as CC10, is a member of the secretoglobin family of small secreted proteins. It is produced by the nonciliated, nonmucous, secretory epithelial clara cells of the pulmonary airways, was first identified in lung lavage by Singh and colleagues. It has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma.

实验方案

Product Specific Protocols
WB protocol for Uteroglobin/CC10 antibody 26909-1-APDownload protocol
IHC protocol for Uteroglobin/CC10 antibody 26909-1-APDownload protocol
IF protocol for Uteroglobin/CC10 antibody 26909-1-APDownload protocol
FC protocol for Uteroglobin/CC10 antibody 26909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

J Immunol

Regulation of MHC Class I Expression in Lung Epithelial Cells during Inflammation.

Authors - Justine Mathé
View Article
ratIHC

Front Pediatr

Impaired FGF10 Signaling and Epithelial Development in Experimental Lung Hypoplasia With Esophageal Atresia.

Authors - Jun Wang
View Article
humanFC

Front Cell Infect Microbiol

BPIFA1 is a secreted biomarker of differentiating human airway epithelium

Authors - Clarissa Clifton
View Article