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  • Featured Product
  • KD/KO Validated

VAC14 Polyclonal antibody

VAC14 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15771-1-AP

Print datasheet

Synonyms

ArPIKfyve, Protein VAC14 homolog, Tax1 binding protein 2, TAX1BP2, TRX, VAC14, Vac14 homolog (S. cerevisiae)

验证数据展示

  • WB analysis of human spleen using 15771-1-AP

    WB analysis of human spleen using 15771-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 15771-1-AP (VAC14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 15771-1-AP

    IHC staining of human skin using 15771-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with VAC14 antibody included
  • IHC staining of human skin using 15771-1-AP

    IHC staining of human skin using 15771-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15771-1-AP (VAC14 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with VAC14 antibody included
  • IP experiment of Jurkat using 15771-1-AP

    IP experiment of Jurkat using 15771-1-AP

    IP result of anti-VAC14 (IP:15771-1-AP, 4ug; Detection:15771-1-AP 1:500) with Jurkat cells lysate 2400ug.

经过测试的应用

Positive WB detected inhuman spleen tissue, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman skin tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy VAC14 Ready-To-Use IHC Kit. VAC14 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15771-1-AP targets VAC14 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen VAC14 fusion protein Ag8415 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Vac14 homolog (S. cerevisiae)
Synonyms ArPIKfyve, Protein VAC14 homolog, Tax1 binding protein 2, TAX1BP2, TRX, VAC14, Vac14 homolog (S. cerevisiae)
Calculated Molecular Weight 88 kDa
Observed Molecular Weight 88 kDa, 65 kDa
GenBank Accession NumberBC007214
Gene Symbol VAC14
Gene ID (NCBI) 55697
RRIDAB_2214753
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08AM6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VAC14 antibody 15771-1-APDownload protocol
IHC protocol for VAC14 antibody 15771-1-APDownload protocol
IP protocol for VAC14 antibody 15771-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Exp Cell Res

Phosphoinositide phosphatase Sac3 regulates the cell surface expression of scavenger receptor A and formation of lipid droplets in macrophages.

Authors - Shin Morioka
  • KD Validated
View Article
WB

Virology

Ebola virus requires phosphatidylinositol (3,5) bisphosphate production for efficient viral entry.

Authors - Shirley Qiu
  • KO Validated
View Article
humanCoIP

Virulence

Coxiella burnetii effector CvpE maintains biogenesis of Coxiella-containing vacuoles by suppressing lysosome tubulation through binding PI(3)P and perturbing PIKfyve activity on lysosomes

Authors - Mingliang Zhao
View Article