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VAChT Polyclonal antibody

VAChT Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27303-1-AP

Print datasheet

Synonyms

SLC18A3

验证数据展示

  • WB analysis using 27303-1-AP

    WB analysis using 27303-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27303-1-AP (VAChT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27303-1-AP targets VAChT in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen VAChT fusion protein Ag25153 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 18 (vesicular acetylcholine), member 3
Synonyms SLC18A3
Calculated Molecular Weight 532 aa, 57 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC007765
Gene Symbol VAChT
Gene ID (NCBI) 6572
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16572
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC18A3 also known as VAChT, is the vesicular amine transporter family member. The SLC18A3 gene encodes a transmembrane protein that transports acetylcholine (ACh) into presynaptic secretory vesicles for release at cholinergic nerve endings in the central and peripheral nervous systems. Mutations in SLC18A3 are associated with congenital myasthenic syndrome(PMID: 27590285). The 68-70 kDa mature glycosylated form of VAChT can be detected (PMID: 14705140).

实验方案

Product Specific Protocols
WB protocol for VAChT antibody 27303-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols