• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

VAMP2 Monoclonal antibody, PBS Only

VAMP2 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG3

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1G7E8

Cat No : 67822-1-PBS

Print datasheet

Synonyms

FLJ11460, SYB2, Synaptobrevin 2, VAMP 2, VAMP2

验证数据展示

  • WB analysis using 67822-1-Ig (same clone as 67822-1-PBS)

    WB analysis using 67822-1-Ig (same clone as 67822-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67822-1-Ig (VAMP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67822-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67822-1-Ig (same clone as 67822-1-PBS)

    IHC staining of mouse brain using 67822-1-Ig (same clone as 67822-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67822-1-Ig (VAMP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67822-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67822-1-Ig (same clone as 67822-1-PBS)

    IHC staining of mouse brain using 67822-1-Ig (same clone as 67822-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67822-1-Ig (VAMP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67822-1-PBS in a different storage buffer formulation.

  • IF Staining of U-87 MG using 67822-1-Ig (same clone as 67822-1-PBS)

    IF Staining of U-87 MG using 67822-1-Ig (same clone as 67822-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using VAMP2 antibody (67822-1-Ig, Clone: 1G7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67822-1-PBS in a different storage buffer formulation.

产品信息

67822-1-PBS targets VAMP2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Mouse, Rat, Pig, Rabbit
Immunogen VAMP2 fusion protein Ag17908 种属同源性预测
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Full Name vesicle-associated membrane protein 2 (synaptobrevin 2)
Synonyms FLJ11460, SYB2, Synaptobrevin 2, VAMP 2, VAMP2
Calculated Molecular Weight 13 kDa
Observed Molecular Weight19 kDa
GenBank Accession NumberBC002737
Gene Symbol VAMP2
Gene ID (NCBI) 6844
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP63027
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

VAMP2 (vesicle-associated membrane protein 2), also named as synaptobrevin 2, is a member of the SNARE (soluble NSF-attachment protein receptor) family proteins. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP2, with a molecular mass of 15-19 kDa, consists of a short N-terminal sequence, a SNARE motif, and a C-terminal transmembrane region. It is required for fast calcium-triggered synaptic vesicle fusion. VAMP2 forms a stable complex with STX1 (syntaxin 1) and SNAP25 (synaptosomal-associated protein 25) during synaptic vesicle fusion (PMID: 16793874). It also forms a distinct complex with synaptophysin. VAMP2 is expressed in nervous system and some non-neuronal tissues, such as skeletal muscle (PMID: 18570252).