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VAMP4 Polyclonal antibody

VAMP4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10738-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

Vesicle-associated membrane protein 4, VAMP-4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse testis tissue, mouse brain tissue, SH-SY5Y cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy VAMP4 Ready-To-Use IHC Kit. VAMP4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10738-1-AP targets VAMP4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 VAMP4 fusion protein Ag1090 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vesicle-associated membrane protein 4
别名 Vesicle-associated membrane protein 4, VAMP-4
计算分子量 16 kDa
观测分子量 18 kDa
GenBank蛋白编号BC007019
基因名称 VAMP4
Gene ID (NCBI) 8674
RRIDAB_2212788
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75379
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VAMP4 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP4 may play a role in trans-Golgi network to endosome transport.

实验方案

Product Specific Protocols
WB protocol for VAMP4 antibody 10738-1-APDownload protocol
IHC protocol for VAMP4 antibody 10738-1-APDownload protocol
IF protocol for VAMP4 antibody 10738-1-APDownload protocol
IP protocol for VAMP4 antibody 10738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,ratWB,IF,IP

J Cell Biol

VAMP4 regulates insulin levels by targeting secretory granules to lysosomes

Authors - Min Li
  • KO Validated
humanWB

J Biol Chem

Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin.

Authors - Wulin Aerbajinai
humanWB

Biochem J

Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44.

Authors - Nazma Malik
ratWB

Biophys Rep

PMID: 30596141

Authors - Min Li
mouseIF

J Biol Chem

Golgi-resident TRIO regulates membrane trafficking during neurite outgrowth.

Authors - Tao Tao
humanWB

J Biol Chem

Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome

Authors - Hana Nůsková
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