验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, Jurkat cells, K-562 cells, pig brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy VAMP4 Ready-To-Use IHC Kit. VAMP4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
67219-1-Ig targets VAMP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Pig, Rat, Mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | Human, Pig, Rat, Mouse |
| 文献引用反应性 | mouse |
| 免疫原 | VAMP4 fusion protein Ag27854 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | vesicle-associated membrane protein 4 |
| 别名 | VAMP 4, VAMP24, VAMP4 |
| 计算分子量 | 16 kDa |
| 观测分子量 | 18 kDa |
| GenBank蛋白编号 | BC007019 |
| 基因名称 | VAMP4 |
| Gene ID (NCBI) | 8674 |
| RRID | AB_2882510 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O75379 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VAMP4 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP4 may play a role in trans-Golgi network to endosome transport.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VAMP4 antibody 67219-1-Ig | Download protocol |
| IHC protocol for VAMP4 antibody 67219-1-Ig | Download protocol |
| IF protocol for VAMP4 antibody 67219-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |