ValRS Polyclonal antibody

ValRS Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15931-1-AP

Print datasheet

Synonyms

G7A, Protein G7a, Valine tRNA ligase, ValRS, Valyl-tRNA synthetase, VARS, VARS1, VARS2



经过测试的应用

Positive WB detected inHL-60 cells
Positive IP detected inHL-60 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15931-1-AP targets ValRS in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ValRS fusion protein Ag8778 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name valyl-tRNA synthetase
Synonyms G7A, Protein G7a, Valine tRNA ligase, ValRS, Valyl-tRNA synthetase, VARS, VARS1, VARS2
Calculated Molecular Weight 1264 aa, 140 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC012808
Gene Symbol ValRS
Gene ID (NCBI) 7407
RRIDAB_2213252
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP26640
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ValRS antibody 15931-1-APDownload protocol
IHC protocol for ValRS antibody 15931-1-APDownload protocol
IP protocol for ValRS antibody 15931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Autoimmun Rev

Two novel anti-aminoacyl tRNA synthetase antibodies: Autoantibodies against cysteinyl-tRNA synthetase and valyl-tRNA synthetase

Authors - Yoshinao Muro
humanWB,IP

Ann Rheum Dis

Mass spectrometry-based identification of new anti-Ly and known antisynthetase autoantibodies

Authors - Jean-Baptiste Vulsteke