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验证数据展示

  • WB analysis using 15931-1-AP

    WB analysis using 15931-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15931-1-AP (ValRS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IP experiment of HL-60 using 15931-1-AP

    IP experiment of HL-60 using 15931-1-AP

    IP result of anti-ValRS (IP:15931-1-AP, 4ug; Detection:15931-1-AP 1:500) with HL-60 cells lysate 3040ug.

  • IHC staining of human tonsillitis using 15931-1-AP

    IHC staining of human tonsillitis using 15931-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15931-1-AP (ValRS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ValRS Polyclonal antibody

ValRS Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 15931-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

Valyl-tRNA synthetase, VARS, EC:6.1.1.9, G7A, Valine tRNA ligase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells, HeLa cells, HepG2 cells, L02 cells
Positive IP detected inHL-60 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15931-1-AP targets ValRS in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IP
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 ValRS fusion protein Ag8778 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 valyl-tRNA synthetase
别名 Valyl-tRNA synthetase, VARS, EC:6.1.1.9, G7A, Valine tRNA ligase
计算分子量 1264 aa, 140 kDa
观测分子量 140 kDa
GenBank蛋白编号BC012808
基因名称 ValRS
Gene ID (NCBI) 7407
RRIDAB_2213252
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP26640
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ValRS antibody 15931-1-APDownload protocol
IHC protocol for ValRS antibody 15931-1-APDownload protocol
IP protocol for ValRS antibody 15931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Autoimmun Rev

Two novel anti-aminoacyl tRNA synthetase antibodies: Autoantibodies against cysteinyl-tRNA synthetase and valyl-tRNA synthetase

Authors - Yoshinao Muro
View Article
humanWB,IP

Ann Rheum Dis

Mass spectrometry-based identification of new anti-Ly and known antisynthetase autoantibodies

Authors - Jean-Baptiste Vulsteke
View Article