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VARS2 Polyclonal antibody

VARS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15776-1-AP

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Synonyms

KIAA1885, Valine tRNA ligase, ValRS, Valyl tRNA synthetase like, VARS2, VARS2L, VARSL



经过测试的应用

Positive WB detected inHeLa cells, human brain tissue, human liver tissue, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15776-1-AP targets VARS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen VARS2 fusion protein Ag8460 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name valyl-tRNA synthetase 2, mitochondrial (putative)
Synonyms KIAA1885, Valine tRNA ligase, ValRS, Valyl tRNA synthetase like, VARS2, VARS2L, VARSL
Calculated Molecular Weight 119 kDa, 57 kDa
Observed Molecular Weight 118 kDa
GenBank Accession NumberBC008844
Gene Symbol VARS2
Gene ID (NCBI) 57176
RRIDAB_2272699
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5ST30
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VARS2(Valine--tRNA ligase, mitochondrial) is also named as KIAA1885, VARS2L, VARSL and belongs to the class-I aminoacyl-tRNA synthetase family. The deduced 993-amino acid protein has an N-terminal mitochondrial targeting signal with a predicted cleavage site after residue 29. VARS2 has characteristics of a class I mitochondrial aminoacyl-tRNA synthetase, including a classical Rossmann fold.

实验方案

Product Specific Protocols
WB protocol for VARS2 antibody 15776-1-APDownload protocol
IHC protocol for VARS2 antibody 15776-1-APDownload protocol
IF protocol for VARS2 antibody 15776-1-APDownload protocol
IP protocol for VARS2 antibody 15776-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

EMBO Mol Med

The isolated carboxy-terminal domain of human mitochondrial leucyl-tRNA synthetase rescues the pathological phenotype of mitochondrial tRNA mutations in human cells.

Authors - Elena Perli
humanIHC

Int J Mol Sci

VARS2 Depletion Leads to Activation of the Integrated Stress Response and Disruptions in Mitochondrial Fatty Acid Oxidation.

Authors - Elham Kayvanpour
  • KD Validated
humanWB

Front Pediatr

Case Report and Review of the Literature: A New and a Recurrent Variant in the VARS2 Gene Are Associated With Isolated Lethal Hypertrophic Cardiomyopathy, Hyperlactatemia, and Pulmonary Hypertension in Early Infancy.

Authors - Katarína Kušíková
humanWB

Metab Brain Dis

Neonatal encephalocardiomyopathy caused by mutations in VARS2.

Authors - Fabian Baertling