验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, Jurkat cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human liver cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15776-1-AP targets VARS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | VARS2 fusion protein Ag8460 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | valyl-tRNA synthetase 2, mitochondrial (putative) |
别名 | Valyl tRNA synthetase like, ValRS, Valine--tRNA ligase, mitochondrial, Valine tRNA ligase, KIAA1885 |
计算分子量 | 119 kDa, 57 kDa |
观测分子量 | 118 kDa |
GenBank蛋白编号 | BC008844 |
基因名称 | VARS2 |
Gene ID (NCBI) | 57176 |
RRID | AB_2272699 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q5ST30 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VARS2(Valine--tRNA ligase, mitochondrial) is also named as KIAA1885, VARS2L, VARSL and belongs to the class-I aminoacyl-tRNA synthetase family. The deduced 993-amino acid protein has an N-terminal mitochondrial targeting signal with a predicted cleavage site after residue 29. VARS2 has characteristics of a class I mitochondrial aminoacyl-tRNA synthetase, including a classical Rossmann fold.
实验方案
Product Specific Protocols | |
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WB protocol for VARS2 antibody 15776-1-AP | Download protocol |
IHC protocol for VARS2 antibody 15776-1-AP | Download protocol |
IF protocol for VARS2 antibody 15776-1-AP | Download protocol |
IP protocol for VARS2 antibody 15776-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
EMBO Mol Med The isolated carboxy-terminal domain of human mitochondrial leucyl-tRNA synthetase rescues the pathological phenotype of mitochondrial tRNA mutations in human cells. | ||
Int J Mol Sci VARS2 Depletion Leads to Activation of the Integrated Stress Response and Disruptions in Mitochondrial Fatty Acid Oxidation.
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Front Pediatr Case Report and Review of the Literature: A New and a Recurrent Variant in the VARS2 Gene Are Associated With Isolated Lethal Hypertrophic Cardiomyopathy, Hyperlactatemia, and Pulmonary Hypertension in Early Infancy. | ||