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VARS2 Polyclonal antibody
VARS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, human brain tissue, human liver tissue, Jurkat cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human liver cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15776-1-AP targets VARS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | VARS2 fusion protein Ag8460 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | valyl-tRNA synthetase 2, mitochondrial (putative) |
Synonyms | KIAA1885, Valine tRNA ligase, ValRS, Valyl tRNA synthetase like, VARS2, VARS2L, VARSL |
Calculated Molecular Weight | 119 kDa, 57 kDa |
Observed Molecular Weight | 118 kDa |
GenBank Accession Number | BC008844 |
Gene Symbol | VARS2 |
Gene ID (NCBI) | 57176 |
RRID | AB_2272699 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q5ST30 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for VARS2 antibody 15776-1-AP | Download protocol |
IHC protocol for VARS2 antibody 15776-1-AP | Download protocol |
IF protocol for VARS2 antibody 15776-1-AP | Download protocol |
IP protocol for VARS2 antibody 15776-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
EMBO Mol Med The isolated carboxy-terminal domain of human mitochondrial leucyl-tRNA synthetase rescues the pathological phenotype of mitochondrial tRNA mutations in human cells. | ||
Int J Mol Sci VARS2 Depletion Leads to Activation of the Integrated Stress Response and Disruptions in Mitochondrial Fatty Acid Oxidation.
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Front Pediatr Case Report and Review of the Literature: A New and a Recurrent Variant in the VARS2 Gene Are Associated With Isolated Lethal Hypertrophic Cardiomyopathy, Hyperlactatemia, and Pulmonary Hypertension in Early Infancy. | ||