VASH2 Polyclonal antibody
VASH2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, HUVEC cells, L02 cells, SW 1990 cells, PC-12 cells |
| Positive IHC detected in | human stomach cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
13303-1-AP targets VASH2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, rat |
| Cited Reactivity | rat |
| Immunogen | VASH2 fusion protein Ag4121 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | vasohibin 2 |
| Synonyms | Vasohibin-like protein, Vasohibin-2, VASHL, Tubulinyl-Tyr carboxypeptidase 2, EC:3.4.17.17 |
| Calculated Molecular Weight | 355 aa, 40 kDa |
| Observed Molecular Weight | 30-34 kDa |
| GenBank Accession Number | BC028194 |
| Gene Symbol | VASH2 |
| Gene ID (NCBI) | 79805 |
| RRID | AB_10642836 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86V25 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VASH2, also named as vasohibin 2, is an important pro-angiogenesis factor in solid tumor. It has been reported that VASH2 is expressed in mononuclear cells mobilized from bone marrow to promote angiogenesis. VASH2 also plays a key role in axon formation. VASH2 is localized as a nuclear type(with 311 amino acid residues) and cytoplasmic type (with 355 amino acid residues and low abundance). Cytoplasmic VASH2 is associated with carcinoma angiogenesis, while nuclear VASH2 may be associated with cell proliferation. This antibody recognizes both the nuclear and cytoplasmic isoforms.(PMID:23615928; 19204325; 31235911; 26177649)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VASH2 antibody 13303-1-AP | Download protocol |
| IHC protocol for VASH2 antibody 13303-1-AP | Download protocol |
| IF protocol for VASH2 antibody 13303-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
