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VASH2 Monoclonal antibody, PBS Only

VASH2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A11D10

Cat no : 67753-1-PBS

Print datasheet

Synonyms

FLJ12505, RP11 275G3.1, VASH2, VASHL, vasohibin 2, Vasohibin like protein

验证数据展示

  • WB analysis using 67753-1-Ig (same clone as 67753-1-PBS)

    WB analysis using 67753-1-Ig (same clone as 67753-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67753-1-Ig (VASH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 67753-1-Ig (same clone as 67753-1-PBS)

    IHC staining of human skin cancer using 67753-1-Ig (same clone as 67753-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67753-1-Ig (VASH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67753-1-PBS in a different storage buffer formulation.

产品信息

67753-1-PBS targets VASH2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen VASH2 fusion protein Ag29985 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vasohibin 2
Synonyms FLJ12505, RP11 275G3.1, VASH2, VASHL, vasohibin 2, Vasohibin like protein
Calculated Molecular Weight 355 aa, 40 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC028194
Gene Symbol VASH2
Gene ID (NCBI) 79805
RRIDAB_2918521
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ86V25
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VASH2, also named as vasohibin 2, is an important pro-angiogenesis factor in solid tumor. It has been reported that VASH2 is expressed in mononuclear cells mobilized from bone marrow to promote angiogenesis. VASH2 also plays a key role in axon formation. VASH2 is localized as a nuclear type(with 311 amino acid residues) and cytoplasmic type (with 355 amino acid residues and low abundance). Cytoplasmic VASH2 is associated with carcinoma angiogenesis, while nuclear VASH2 may be associated with cell proliferation. 67753-1-Ig antibody recognizes both the nuclear and cytoplasmic isoforms. (PMID:23615928; 19204325; 31235911; 26177649)