- Featured Product
- KD/KO Validated
VAT1 Polyclonal antibody
VAT1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | C6 cells, Jurkat cells, MCF-7 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human gliomas tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy VAT1 Ready-To-Use IHC Kit. VAT1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
22016-1-AP targets VAT1 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human |
| Immunogen | VAT1 fusion protein Ag16652 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | vesicle amine transport protein 1 homolog (T. californica) |
| Synonyms | VAT1, VATI |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42-50 kDa |
| GenBank Accession Number | BC015041 |
| Gene Symbol | VAT1 |
| Gene ID (NCBI) | 10493 |
| RRID | AB_11182282 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99536 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VAT1 antibody 22016-1-AP | Download protocol |
| IHC protocol for VAT1 antibody 22016-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Immunol Immunother Novel roles of VAT1 expression in the immunosuppressive action of diffuse gliomas.
| ||
Oncol Rep High expression of VAT1 is a prognostic biomarker and predicts malignancy in glioblastoma. |
