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验证数据展示

  • WB analysis using 22016-1-AP

    WB analysis using 22016-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22016-1-AP (VAT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 22016-1-AP

    IHC staining of human gliomas using 22016-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 22016-1-AP (VAT1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with VAT1 antibody included
  • IHC staining of human gliomas using 22016-1-AP

    IHC staining of human gliomas using 22016-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 22016-1-AP (VAT1 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with VAT1 antibody included
  • Featured Product
  • KD/KO验证

VAT1 Polyclonal antibody

VAT1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22016-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, rat, mouse

应用

WB, IHC, ELISA

别名

VAT1, VATI

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inC6 cells, Jurkat cells, MCF-7 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman gliomas tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy VAT1 Ready-To-Use IHC Kit. VAT1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22016-1-AP targets VAT1 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, rat, mouse
文献引用反应性human
免疫原 VAT1 fusion protein Ag16652 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vesicle amine transport protein 1 homolog (T. californica)
别名 VAT1, VATI
计算分子量 42 kDa
观测分子量 42-50 kDa
GenBank蛋白编号BC015041
基因名称 VAT1
Gene ID (NCBI) 10493
RRIDAB_11182282
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99536
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VAT1 antibody 22016-1-APDownload protocol
IHC protocol for VAT1 antibody 22016-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,WB

Cancer Immunol Immunother

Novel roles of VAT1 expression in the immunosuppressive action of diffuse gliomas.

Authors - Pei Yang
  • KD Validated
View Article
humanWB

Oncol Rep

High expression of VAT1 is a prognostic biomarker and predicts malignancy in glioblastoma.

Authors - Xia Shan
View Article