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VAV2 Polyclonal antibody

VAV2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 21924-1-AP

Print datasheet

Synonyms

VAV-2, VAV 2

验证数据展示

  • WB analysis using 21924-1-AP

    WB analysis using 21924-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 21924-1-AP

    IHC staining of human pancreas using 21924-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 21924-1-AP (VAV2 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with VAV2 antibody included
  • IHC staining of human pancreas using 21924-1-AP

    IHC staining of human pancreas using 21924-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 21924-1-AP (VAV2 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with VAV2 antibody included
  • IHC staining of human kidney using 21924-1-AP

    IHC staining of human kidney using 21924-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21924-1-AP (VAV2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VAV2 antibody included
  • IHC staining of human kidney using 21924-1-AP

    IHC staining of human kidney using 21924-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21924-1-AP (VAV2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VAV2 antibody included
  • WB analysis using 21924-1-AP

    WB analysis using 21924-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 21924-1-AP

    IP experiment of HeLa using 21924-1-AP

    IP result of anti-VAV2 (IP:21924-1-AP, 4ug; Detection:21924-1-AP 1:2000) with HeLa cells lysate 1520 ug.

  • IF Staining of HeLa using 21924-1-AP

    IF Staining of HeLa using 21924-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VAV2 antibody (21924-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, A431 cells, NIH/3T3 cells, HeLa cells, mouse liver tissue, rat liver tissue, SH-SY5Y cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy VAV2 Ready-To-Use IHC Kit. VAV2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21924-1-AP targets VAV2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen VAV2 fusion protein Ag16458 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name vav 2 guanine nucleotide exchange factor
Synonyms VAV-2, VAV 2
Calculated Molecular Weight 839 aa, 97 kDa
Observed Molecular Weight 101 kDa
GenBank Accession NumberBC132967
Gene Symbol VAV2
Gene ID (NCBI) 7410
RRIDAB_11182283
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52735
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VAV2 antibody 21924-1-APDownload protocol
IHC protocol for VAV2 antibody 21924-1-APDownload protocol
IF protocol for VAV2 antibody 21924-1-APDownload protocol
IP protocol for VAV2 antibody 21924-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

FASEB J

Mesenchymal stem cells promote endothelial progenitor cell migration, vascularization, and bone repair in tissue-engineered constructs via activating CXCR2-Src-PKL/Vav2-Rac1.

Authors - Zhilin Li
View Article
humanWB,IP,IF

J Nanobiotechnology

Proangiogenic effect and underlying mechanism of holmium oxide nanoparticles: a new biomaterial for tissue engineering

Authors - Yuxiao Luo
  • KD Validated
View Article
WB

Nat Commun

Neutrophil extracellular traps-inspired DNA hydrogel for wound hemostatic adjuvant

Authors - Rui Ye
View Article