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VAV2 Monoclonal antibody

VAV2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3F11B5

Cat no : 67108-1-Ig

Print datasheet

Synonyms

VAV 2, VAV2

验证数据展示

  • WB analysis of fetal human brain using 67108-1-Ig

    WB analysis of fetal human brain using 67108-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67108-1-Ig (VAV2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 67108-1-Ig

    IF Staining of A431 using 67108-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using VAV2 antibody (67108-1-Ig, Clone: 3F11B5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • IF Staining of HUVEC using 67108-1-Ig

    IF Staining of HUVEC using 67108-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using VAV2 antibody (67108-1-Ig, Clone: 3F11B5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

经过测试的应用

Positive WB detected infetal human brain tissue, A431 cells, HeLa cells, HEK-293 cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inA431 cells, HeLa cells, HUVEC cells
Planning an IHC experiment? We recommend our IHCeasy VAV2 Ready-To-Use IHC Kit. VAV2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67108-1-Ig targets VAV2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, pig, mouse, rat
Immunogen VAV2 fusion protein Ag17988 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name vav 2 guanine nucleotide exchange factor
Synonyms VAV 2, VAV2
Calculated Molecular Weight 839 aa, 97 kDa
Observed Molecular Weight 101 kDa
GenBank Accession NumberBC132967
Gene Symbol VAV2
Gene ID (NCBI) 7410
RRIDAB_2882412
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52735
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VAV2 antibody 67108-1-IgDownload protocol
IF protocol for VAV2 antibody 67108-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols