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VAV3 Polyclonal antibody

VAV3 Polyclonal Antibody for WB, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat No : 30291-1-AP

Print datasheet

Synonyms

VAV 3, Guanine nucleotide exchange factor VAV3

验证数据展示

  • WB analysis using 30291-1-AP

    WB analysis using 30291-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30291-1-AP (VAV3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 30291-1-AP

    IP experiment of Jurkat using 30291-1-AP

    IP result of anti-VAV3 (IP:30291-1-AP, 4ug; Detection:30291-1-AP 1:8000) with Jurkat cells lysate 1560 ug.

  • FC experiment of Jurkat using 30291-1-AP

    FC experiment of Jurkat using 30291-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human VAV3 (30291-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inJurkat cells, THP-1 cells
Positive IP detected inJurkat cells
Positive FC (Intra) detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30291-1-AP targets VAV3 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), IP, ELISA Application Description
Tested Reactivity human
Immunogen VAV3 fusion protein Ag33022 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name vav 3 guanine nucleotide exchange factor
Synonyms VAV 3, Guanine nucleotide exchange factor VAV3
Calculated Molecular Weight98kd
Observed Molecular Weight 98 kDa
GenBank Accession NumberNM_006113
Gene Symbol VAV3
Gene ID (NCBI) 10451
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKW4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vav3, a member of Dbl family which is also the activator of GTP kinase of Rho family, can activate the expression of RhoA, Racl and Cdc42 (PMID:28285969). Vav3 is associated with tumor growth, apoptosis, invasion, metastasis and angiogenesis (PMID:28285969). Vav3 is a multiple function protein with both signaling molecule and coactivator activities. Vav3 oncogene is overexpressed in androgen-independent prostate cancer cells relative to their androgen-dependent counterparts and contribute to development of hormone refractory prostate cancer (PMID:21455584).

实验方案

Product Specific Protocols
WB protocol for VAV3 antibody 30291-1-APDownload protocol
IP protocol for VAV3 antibody 30291-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols